相关实验视频

Updated: Jan 18, 2026

Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection

Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection

Published on: February 16, 2020

抗原特异化基因CCL3作为生物标志物,用于区分最近和远程结核病感染

Chunyan Chang^1,2, Zichun Ma¹, Weicong Ren¹

¹Department of Bacteriology and Immunology, Beijing Chest Hospital, Capital Medical University/Beijing Tuberculosis and Thoracic Tumor Research Institute, Postal No 9, Beiguan Street, Tongzhou District, Beijing, 101149, People's Republic of China.

|May 25, 2025

概括

识别最近的结核病 (TB) 感染对于控制结核病至关重要. 使用CCL3的新测定方法显示,在潜在结核病感染个体中准确检测最近的结核病感染具有前途.

科学领域:

传染性疾病传染性疾病
免疫学免疫学免疫学
生物标志物发现发现

背景情况:

控制结核病 (TB) 取决于识别最近的感染和实施结核病预防疗法 (TPT).
目前的诊断很难确定患有最近感染活动性结核病的高风险人群.
这项研究寻求新的生物标志物,以区分最近的结核病感染与远程结核病感染.

研究的目的:

探索候选蛋白质生物标志物,以区分最近的结核病感染与远程结核病感染.
为了确定可靠的指标,高风险的个人需要及时的结核病预防治疗.

主要方法:

招募结核病患者的亲密接触者进入查 (n=45) 和验证 (n=76) 队列.
在使用Olink蛋白质组和Luminex测定剂的Mycobacterium tuberculosis (Mtb) 抗原刺激血中测量了与炎症相关的蛋白质生物标志物.
分析了最近和远程结核病感染组之间生物标志物水平的差异.

主要成果:

在最近和远程结核病感染组之间发现了CCL3,CCL20,CCL23和TNF-α的mtb抗原刺激血水平的显著差异.
CCL3,CCL20和TNF-α水平预测了最近的结核病感染,CCL3显示了最高的诊断性能 (AUC=0.859,敏感性=86.4%,特异性=75%).
观察到NF-κB信号通路的潜在激活,有助于差异性免疫细胞反应.

结论:

使用CCL3进行的Mtb抗原特异性检测显示出卓越的诊断性能.
这种基于CCL3的测定方法可以显著提高诊断准确度,以识别潜在结核病感染 (LTBI) 患者最近的结核病感染.

关键词:

生物标志物生物标志物潜在的结核病感染.蛋白质组学是指蛋白质组学.最近感染的感染情况

更多相关视频

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo

Published on: August 11, 2018

Separation and Fractionation of Culture Filtrate Proteins (CFPs) from Mycobacterium tuberculosis

Separation and Fractionation of Culture Filtrate Proteins (CFPs) from Mycobacterium tuberculosis

Published on: July 11, 2025

相关概念视频

Pulmonary Tuberculosis IV

Pulmonary Tuberculosis IV

Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...

Pulmonary Tuberculosis III

Pulmonary Tuberculosis III

Tuberculosis (TB) is a contagious infection primarily affecting the lung parenchyma but which can also affect other body parts. TB can be classified based on disease development, presentation, and the affected anatomical site.
The first classification is based on the development of the disease, and it includes the following categories: