罗利尼特林是抗微生物活性和Pseudomonas chlororaphis菌株氨酸生物合成的组成部分
Mahnoor Zameer1, Izzah Shahid2, Deeba Noreen Baig1
1Kauser Abdulla Malik School of Life Sciences, Forman Christian College (A Chartered University), Ferozepur Road, Lahore, 54600, Pakistan.
World journal of microbiology & biotechnology
|May 25, 2025
概括
这项研究表明,抑制Pseudomonas chlororaphis中的pyrrolnitrin (PRN) 生产显著降低了其对抗性质. 纯化PRN具有强大的抗真菌,抗菌和抗癌活性,突出显示其作为生物控制和治疗剂的潜力.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 伪omonas物种产生各种各样的代谢物,包括pyrrolnitrin (PRN),一种具有抗生素特性的烯.
- 在Pseudomonas chlororaphis菌株对抗能力中PRN的作用尚未完全理解.
研究的目的:
- 为了研究抑制皮罗利尼特林生产对Pseudomonas chlororaphis.的对抗性质的影响.
- 为了评估纯化二的抗微生物和细胞毒性潜力.
主要方法:
- 通过使用自杀载体来抑制prnA基因,产生了Pseudomonas chlororaphis突变.
- 使用高性能液态染色学 (HPLC) 量化罗丁和酶的生产.
- 评估净化PRN对真菌和细菌病原体的抗微生物活性及其对癌症细胞系的抗增殖作用.
主要成果:
- 突变者表现出显著减少的罗利特林和氨酸-1-碳酸酸 (PCA) 生产.
- 纯化的PRN显示出强大的抑制真菌子形成 (Alternaria alternata,Aspergillus niger).
- PRN对Salmonella enterica,Bacillus cereus和Staphylococcus sp显著的抗菌活性,以及对HepG-2和SF767癌细胞系的抗增殖作用.
结论:
- 对于 Pseudomonas chlororaphis.的全部对抗潜力而言,罗利特林的产生至关重要.
- 纯化铁酸具有广泛的抗微生物和细胞毒性活动.
- 作为植物病原体的生物控制剂和作为治疗化合物,罗利尼是有前途的.
相关概念视频
Gene Regulation in Microbial Communities: Quorum Sensing
99
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
99
Aryldiazonium Salts to Azo Dyes: Diazo Coupling
3.1K
The reaction of weakly electrophilic aryldiazonium (also called arenediazonium) salts with highly activated aromatic compounds leads to the formation of products with an —N=N— link, called an azo linkage. This reaction, presented in Figure 1, is known as diazo coupling and occurs without the loss of the nitrogen atoms of the aryldiazonium salt. Highly activated aromatic compounds such as phenols or arylamines favor the diazo coupling reaction. The coupling generally occurs at the...
3.1K
Biosynthesis of Nucleic Acids
217
Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
217
Basicity of Heterocyclic Aromatic Amines
6.3K
Heterocyclic amines, where the N atom is a part of an alicyclic system, are similar in basicity to alkylamines. Interestingly, the heterocyclic amine having a nitrogen atom as part of an aromatic ring has much less basicity than its corresponding alicyclic counterpart. For this reason, as presented in Figure 1, piperidine (pKb = 2.8) is significantly more basic than pyridine (pKb = 8.8).
6.3K
Types of RNA
66.7K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
66.7K
Nucleophilic Aromatic Substitution of Aryldiazonium Salts: Aromatic SN1
2.2K
Treating arylamines with nitrous acid gives aryldiazonium salts that are effective substrates in nucleophilic aromatic substitution reactions. The diazonio group in these salts can be easily displaced by different nucleophiles, yielding a wide variety of substituted benzenes. The leaving group departs as nitrogen gas, and this easy elimination is the driving force for the substitution reaction.
In the Sandmeyer reaction, for example, the diazonio group is replaced by a chloro, bromo,...
In the Sandmeyer reaction, for example, the diazonio group is replaced by a chloro, bromo,...
2.2K


![Cercosporin-Photocatalyzed [4+1]- and [4+2]-Annulations of Azoalkenes Under Mild Conditions](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F60786.jpg&w=3840&q=50)