相关实验视频

Updated: Jun 30, 2026

Large Scale Zebrafish-Based In vivo Small Molecule Screen

Large Scale Zebrafish-Based In vivo Small Molecule Screen

Published on: December 30, 2010

小黑破坏了斑马鱼幼虫的血管发育和血液形成

Zhuyi Zhang¹, Hua Chang^2,3,4, Yu Hao¹

¹Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Department of Toxicology, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China.

Environmental science & technology

|May 26, 2025

概括

小型黑 (S-BP) 暴露会导致斑马鱼的发育毒性,影响血管生长和血细胞形成. 这项研究强调了与环境中的二维纳米材料相关的风险.

科学领域:

环境毒理学环境毒理学
纳米材料的安全性
斑马鱼的发育生物学

背景情况:

黑 (BP) 是一种新的二维材料,有新兴的应用,但其环境风险尚不清楚.
潜在的生态和健康问题源于BP无意中释放到环境中.
了解小型黑 (S-BP) 的毒性对于风险评估至关重要.

研究的目的:

评估S-BP在低度下对斑马鱼的发育毒性.
研究S-BP对血管发育和血液形成的特定影响.
为了确定敏感的生物点和S-BP毒性的基础分子机制.

主要方法:

使用野生类型和转基因斑马鱼进行毒性评估.
斑马鱼幼虫暴露于S-BP,其度在0.025至0.2毫克/升之间.
采用血管架构分析和转录组分析来研究S-BP效应.

主要成果:

暴露于S-BP导致发育缺陷,包括未膨胀的游泳膀和心率降低.
观察到血管发育受损,特别是在常心静脉 (CCV) 中.
红细胞和红细胞 (RBC) 流速受到干扰,血管新生和血液形成途径的基因表达发生改变.

结论:

低度的S-BP诱导斑马鱼显著的血管和血液毒性.
常心静脉 (CCV) 和红细胞 (RBC) 被确定为S-BP毒性的敏感目标.
这项研究为像黑这样的二维纳米材料的环境风险评估提供了关键数据.

关键词:

发展性毒性发展性毒性血液形成血液形成纳米材料的使用方法小黑色的小黑色.血管毒性血管毒性

更多相关视频

Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells HSPCs in Developing Zebrafish Embryos

Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells HSPCs in Developing Zebrafish Embryos

Published on: November 30, 2017

Small Molecule Screening and Toxicity Testing in Early-stage Zebrafish Larvae

Small Molecule Screening and Toxicity Testing in Early-stage Zebrafish Larvae

Published on: March 7, 2025

相关概念视频

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...