与OsbZIP61相互作用的OsRF2b通过中的异构体调节使用效率和谷物产量
Cuiping Li1, Huan Wen1, Yuanyuan Wu1
1College of Life Sciences, Chongqing Normal University, Chongqing, China.
Plant biotechnology journal
|May 26, 2025
概括
研究人员确定了OsRF2b和OsbZIP61两种转录因子,它们对利用效率 (NUE) 和谷物产量产生负面调节. 了解这些因素为改善米育种提供了基因基础,以获得更好的产量和NUE.
科学领域:
- 植物分子生物学 植物分子生物学
- 农作物科学 农作物科学
- 遗传学 遗传学 是一个
背景情况:
- (N) 对作物生长至关重要,使得利用效率 (NUE) 成为提高谷物产量和质量的关键目标.
- 识别NUE的遗传调节者对于开发增强作物品种至关重要.
研究的目的:
- 确定和描述涉及调节使用效率 (NUE) 和大米谷物产量的转录因子.
- 阐明这些因素影响NUE和产量的分子机制.
主要方法:
- 使用RT-qPCR进行基因表达分析.
- 蛋白质-DNA相互作用测试 (酵母一种混合,EMSA).
- 蛋白质与蛋白质相互作用试验 (BiFC,LCI).
- 对转基因大米谱系和突变物的分析.
主要成果:
- OsRF2b和OsbZIP61被确定为大米NUE和谷物产量的负调节剂.
- OsRF2b直接与OsNRT1.1B的促进体结合,抑制其表达.
- OsRF2b与OsbZIP61相互作用,形成一个影响NUE的异构体.
- 过度表达OsRF2b降低了含量和NUE,而它的突变增加了它们.
结论:
- bZIP转录因子OsRF2b与OsbZIP61相互作用,对米NUE和谷物产量产生负面调节.
- 这种调节通过直接结合OsNRT1.1B促进体并抑制其表达而发生.
- 这些发现为NUE的遗传机制提供了洞察力,并为米育种提供了目标.
更多相关视频
11:33Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer
Published on: October 14, 2022
1.7K
07:43Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
Published on: October 6, 2020
12.7K
相关概念视频
01:22Inorganic Nitrogen Assimilation
120
Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
120
01:23Transcriptional Regulation: Riboswitches
128
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
128
01:17Gene Regulation During Sporulation
88
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
88
02:35Key Elements for Plant Nutrition
22.2K
Like all living organisms, plants require organic and inorganic nutrients to survive, reproduce, grow and maintain homeostasis. To identify nutrients that are essential for plant functioning, researchers have leveraged a technique called hydroponics. In hydroponic culture systems, plants are grown—without soil—in water-based solutions containing nutrients. At least 17 nutrients have been identified as essential elements required by plants. Plants acquire these elements from the...
22.2K
01:56Riboswitches
8.7K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.7K