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相关概念视频

Chemical Shift: Internal References and Solvent Effects01:17

Chemical Shift: Internal References and Solvent Effects

833
In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
833
NMR Spectroscopy: Chemical Shift Overview01:15

NMR Spectroscopy: Chemical Shift Overview

1.7K
The position of the absorption signal of a sample is reported relative to the position of the signal of tetramethylsilane (TMS), which is added as an internal reference while recording spectra. The difference between the absorption frequencies of the sample and TMS (in Hz) is divided by the spectrometer operating frequency (in MHz) to obtain a dimensionless quantity called the chemical shift. It is reported on the δ (delta) scale and expressed in parts per million.
For instance, the proton...
1.7K
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

934
Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
934
NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

1.3K
NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
1.3K
Applications Of NMR In Biology01:25

Applications Of NMR In Biology

4.0K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
4.0K
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.1K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.1K

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药物分析中的定量固态NMR光谱学 (qSSNMR)

Zhaoxi Zheng1, Kang Chen2, Yang Liu3

  • 1Department of Chemistry, Brandeis University, Waltham, Massachusetts, USA.

Magnetic resonance in chemistry : MRC
|May 26, 2025
PubMed
概括

定量固态NMR (qSSNMR) 增强了药物开发的药物分析. 进步提高了复杂混合物的精度,速度和分析,解决了采用挑战.

关键词:
复杂的药物配方复杂的药物配方.药品分析 药品分析在QSSNMR中,QSSNMR是指QSSNMR.定量固态NMR的固态NMR是什么固态特征的固态特征.

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科学领域:

  • 分析化学 分析化学
  • 制药科学 制药科学
  • 材料科学 材料科学 材料科学

背景情况:

  • 药物分析对于药物开发和质量控制至关重要.
  • 确保药物的安全性和有效性依赖于精确的分析方法.
  • 定量固态NMR (qSSNMR) 是固体药物配方的重要技术.

研究的目的:

  • 审查定量固态NMR (qSSNMR) 的演变和进展.
  • 为了突出技术上的改进,在qSSNMR药物应用.
  • 讨论 qSSNMR 在分析复杂药物混合物的应用.

主要方法:

  • 关于定量固态NMR (qSSNMR) 技术的文献综述.
  • 对检测极限,分辨率和吞吐量方面的改进进行分析.
  • 在药物分析中探索qSSNMR应用.

主要成果:

  • 在qSSNMR检测极限和分辨率上有显著的改进.
  • 增强高吞吐量功能,用于更快的分析.
  • 在分析复杂的药物混合物中成功应用qSSNMR.

结论:

  • qSSNMR技术已经显著发展,为药物分析提供了增强的能力.
  • 正在进行的自动化和用户友好的软件努力旨在增加qSSNMR的采用.
  • qSSNMR是药物开发和质量保证的强大工具.