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在败血症引起的急性肺损伤中,Circ_0008285通过miR-375/MAPK14轴调节巨细胞两极分化

Chen Li¹, Jianhua Liu¹, Gaixia Feng¹

¹Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Hebei North University, No. 12 Changqing Road, Qiaoxi District, Zhangjiakou, 075000, Hebei, China.

Journal of molecular histology

|May 26, 2025

概括

循环RNAcirc_0008285通过菌miR-375并影响MAPK14.4来调节败血症引起的急性肺损伤 (ALI) 中的巨细胞两极分化. 这一发现为ALI提供了新的治疗策略.

科学领域:

分子生物学分子生物学
免疫学免疫学免疫学
病理学病理学病理学

背景情况:

败血症引起的急性肺损伤 (ALI) 是一种由巨细胞两极分化为特征的严重疾病.
循环RNAcirc_0008285已与各种疾病有关,其在败血症-ALI中的作用需要研究.

研究的目的:

阐明circ_0008285在败血症诱导的ALI中的调节作用和潜在机制.
为了研究circ_0008285,miR-375和MAPK14之间的相互作用,在败血症-ALI.

主要方法:

在体外 (用LPS治疗的RAW264.7细胞) 和体内 (C57BL/6小鼠) 建立败血症诱导的ALI.模型.
使用qRT-PCR,西斑,CCK-8试验,流细胞计,双露西法酶记者试验,HE染色和TUNEL染色来分析分子和细胞变化.
研究了circ_0008285敲击对巨细胞极化标记物和肺组织损伤的影响.

主要成果:

在LPS诱导的细胞中,Circ_0008285和MAPK14的上调,而miR-375的下调.
Knockdown of circ_0008285减少了促炎性细胞因子 (IL-1β,IL-6,TNF-α) 和M1标记物 (iNOS,CD86),同时增加了抗炎性标记物 (CD206,Arg1) 在体外和体内.
Circ_0008285直接对miR-375进行海绵,miR-375针对MAPK14,最终改善了败血症-ALI中的肺损伤和巨细胞两极分化.

结论:

Circ_0008285通过培养miR-375和提高MAPK14的调节来促进败血症诱导的ALI,从而推动巨细胞的两极分化.
针对circ_0008285/miR-375/MAPK14轴为败血症诱导的ALI提供了一个潜在的治疗途径.

关键词:

急性肺损伤是什么?急性肺损伤是什么?巨细胞的两极分化败血症这是一种败血症.环_0008285 周围的世界据报道,米R-375/MAPK14与MAPK14之间的关系是:

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