用Yishen进行结肠透析,通过调节SIRT1通路来改善慢性功能衰竭肠膜上皮细胞的自性疾病
Yan-Jun Fan1, Jing-Ai Fang1, Su-Fen Li2,3
1Department of Nephrology, the First Hospital of Shanxi Medical University, Taiyuan, 030001, China.
Chinese journal of integrative medicine
|May 26, 2025
概括
伊申开结肠透析通过调节SIRT1表达来改善慢性功能衰竭 (CRF) 鼠的自性障碍和肠壁损伤. 这种疗法为CRF相关并发症提供了潜在的治疗方法.
科学领域:
- 腎臟病學 (nephrology) 是一種醫學專業.
- 胃肠病学 胃肠病学
- 细胞生物学 细胞生物学
背景情况:
- 慢性功能衰竭 (CRF) 与肠道上皮细胞的自性障碍有关.
- 肠粘膜屏障损伤是CRF的一种并发症.
- 伊申 (Yishen Decoction,简称YS) 是一种中国传统医药,具有潜在的治疗效果.
研究的目的:
- 为了研究结肠透析与Yishen (YS) 的机制,以改善慢性功能衰竭 (CRF) 鼠的肠上皮细胞的自性障碍.
- 探索SIRT1在这个过程中的作用.
主要方法:
- 使用腺素建立的CRF大鼠模型.
- 结肠透析与YS治疗给CRF大鼠.
- 测量了血清肌素 (SCr) 和血液尿素 (BUN) 的水平.
- 脏和结肠组织的组织病理学检查.
- 通过电子显微镜观察自细胞.
- 在体外研究中,使用T84细胞进行了各种治疗 (尿素模型,YS,拉巴胺素,3-MA,白醇).
- 对基因和蛋白质表达的RT-PCR和西部斑点分析 (ZO-1,克劳丁-1,SIRT1,LC3,Beclin-1).
主要成果:
- 在CRF大鼠中,YS结肠透析降低了SCr和BUN水平,并减轻了组织损伤.
- 在体内,YS治疗增加了SIRT1,ZO-1,Claudin-1,Beclin-1和LC3II/I的表达.
- 在体外,尿素模型降低了SIRT1,ZO-1和Claudin-1,同时增加了Beclin-1和LC3II/I.
- 在实验室中,YS治疗逆转了这些变化.
- SIRT1激活剂 (复星) 改善了自和肠道屏障标记物.
结论:
- 用Yishen Decoction进行结肠透析可以改善自性乱,并修复CRF的肠粘膜屏障损伤.
- 该机制涉及肠道上皮细胞中SIRT1表达的调节.
相关概念视频
01:27Dialysis
1.2K
Renal failure occurs when the kidneys lose their ability to filter waste products from the blood effectively. It can be classified into two types: acute renal failure (ARF) and chronic renal failure (CRF).
Acute kidney injury develops suddenly and can be caused by pre-renal causes (e.g., hypovolemia, shock), intrinsic renal causes (e.g., acute tubular necrosis), or post-renal causes (e.g., urinary obstruction). In contrast, chronic renal failure progresses gradually over time and is often...
Acute kidney injury develops suddenly and can be caused by pre-renal causes (e.g., hypovolemia, shock), intrinsic renal causes (e.g., acute tubular necrosis), or post-renal causes (e.g., urinary obstruction). In contrast, chronic renal failure progresses gradually over time and is often...
1.2K
01:28Chronic Kidney Disease III: Interprofessional Care
346
Chronic kidney disease (CKD) requires collaborative and comprehensive management. CKD progresses through stages and can lead to end-stage kidney disease (ESKD) if untreated. Interprofessional collaboration and patient education are crucial, enabling patients to manage their health and improve their quality of life.Diagnostic approach for chronic kidney diseaseThe diagnosis of CKD primarily focuses on the glomerular filtration rate (GFR), which assesses kidney function by measuring how well...
346
01:25Chronic Kidney Disease I: Introduction
573
Chronic Kidney Disease (CKD) arises when the kidneys progressively lose their ability to function, ultimately leading to end-stage renal disease. At this advanced stage, the kidneys can no longer filter waste or maintain essential body functions, requiring renal replacement therapy (RRT) through dialysis or a kidney transplant for survival.Early-stage chronic kidney disease and detection challengesIn CKD's early stages, symptoms often remain absent because healthy nephrons compensate for...
573
01:20Acute Kidney Injury V: Interprofessional Care
297
Acute Kidney Injury (AKI) requires a collaborative healthcare approach to restore renal function and prevent complications. Essential management strategies involve monitoring fluid and electrolyte balance, adjusting medications, initiating dialysis when necessary, and providing nutritional support.Fluid and Electrolyte ManagementFluid Monitoring: Regularly monitoring body weight, central venous pressure, and urine output helps detect fluid imbalances early. Patient intake and output are...
297