在Lacticaseibacillus rhamnosus GG的推动下,阿尔金因代谢的重塑减轻了肠道屏障功能障碍
Jayson M Antonio1, Yue Liu2, Panan Suntornsaratoon1,3
1Department of Pharmacology, Physiology and Neurosciences, New Jersey Medical School, Rutgers University, Newark, New Jersey, United States.
益生菌Lacticaseibacillus rhamnosus GG通过促进氨酸代谢,特别是氨酸酸酶 (ASL) 表达,当饮食托足够时,增强肠道屏障功能. 这种益生菌机制可能为炎症性肠道疾病提供治疗潜力.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 代谢途径 代谢途径
背景情况:
- 炎症性肠道疾病 (IBD) 和肠道屏障功能障碍与改变的酸盐和氨酸代谢有关.
- 肠道屏障完整性受到干扰或恢复的确切机制尚不清楚.
研究的目的:
- 研究益生菌Lacticaseibacillus rhamnosus GG (LGG) 在通过饮食营养代谢调节肠道屏障功能的作用.
- 阐明由LGG影响的特定代谢途径,特别是涉及氨酸,以及它们对肠道平衡的影响.
主要方法:
- 使用了由德克斯硫酸诱导的实验性结肠炎的小鼠模型.
- 给 Lacticaseibacillus rhamnosus GG (LGG) 和评估其对肠道氨酸酸酶 (ASL) 表达和血清代谢物水平的影响.
- 分析了紧结蛋白和氧化合成酶 (NOS2) 的基因表达.
- 在IBD患者中检查了代谢概况.
主要成果:
- LGG殖民显著提高了肠道氨酸酸酶 (ASL) 表达的调节,增强了氨酸生物合成和肠道屏障功能.
- 实验性结肠炎导致ASL表达减少和血清中阿尔吉尼诺糖酸盐的升高,这种代谢物与紧结基因表达相反相关.
- 来自LG的醇和氨酸代谢物促进了ASL和NOS2的表达,将微生物活动与氧化生产和上皮健康联系起来.
- 患有IBD的患者表现出增加的ASS1和减少的ASL表达,这表明阿金合成中的代谢瓶.
结论:
- 乳酸菌cillus rhamnosus GG,与饮食中的酸协同作用,通过上调ASL来增强肠道屏障的完整性,ASL是氨酸生物合成中的关键酶.
- 在实验性结肠炎中,ASL的下调和血清中氨酸酸的升高表明IBD的潜在治疗标.
- 这些发现揭示了一种新的益生菌媒介机制,将饮食中的托,宿主阿基尼因代谢和肠道屏障稳定联系在一起,为精确的益生菌策略提供了基础.
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