开发一种基于黄金纳米粒子的色度测试方法,以快速检测总细菌数量和大肠杆菌,使用分子杂交方法
Juan Du1, Ziqi Huang2, Zongshuang Li2
1College of Food Science and Engineering, Henan University of Technology, Zhengzhou, China; Postdoctoral Programme, Henan Doyoo(Group) Industrial Co., Ltd., Hebi, China; College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou,China; Key Laboratory of Cold Chain Food Processing and Safety Control (Zhengzhou University of Light Industry), Ministry of Education, Zhengzhou, China.
Journal of microbiological methods
|May 26, 2025
概括
一种新的金纳米粒子测定方法快速检测食品中的总细菌计数 (TBC) 和大肠杆菌. 这种简单的,不需要设备的方法提供了高灵敏度的现场食品安全测试.
科学领域:
- 食品微生物学 食品微生物学
- 纳米技术纳米技术
- 生物传感器是一种生物传感器.
背景情况:
- 总细菌计数 (TBC) 和大肠杆菌是食品微生物质量和便污染的关键指标.
- 目前的检测方法往往耗时,需要专门的设备.
- 标准化微生物学标准对于确保全球食品安全至关重要.
研究的目的:
- 开发一种快速,灵敏和现场色度测试方法,用于检测食品中的TBC和大肠杆菌.
- 为了利用金纳米粒子 (AuNPs) 和分子杂交来产生信号.
- 建立一个具有成本效益和可访问的食品安全监测方法.
主要方法:
- 用醇修饰的原料 (SH-27F和SH-lacZF) 与AuNPs的结合.
- 使用盐诱导的AuNP聚合作为分子杂交的指标.
- 使用智能手机应用程序 (ColorPicker) 进行定量色度分析.
主要成果:
- 该试验通过视觉检查实现了结核病检测极限为10^2 CFU/mL,大肠杆菌检测极限为10^1 CFU/mL.
- 紫外光谱分析给出了10^1 CFU/mL的检测极限.
- 在颜色信号和大肠杆菌度 (10^1-10^4 CFU/mL) 之间观察到线性关系.
结论:
- 开发的基于AuNPs的色度测试方法快速,简单,灵敏,不需要培养丰富或昂贵的设备.
- 这种方法显示出在现场检测食品中的TBC和大肠杆菌的巨大潜力.
- 该战略为开发其他致病微生物的类似色度探针提供了一个框架.
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