相关实验视频

Updated: Sep 20, 2025

Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos

Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos

Published on: April 28, 2017

一个染色体层次的基因组组装的甜菜网虫,Loxostege sticticalis 林奈 (Lepidoptera:Pyralidae) 的

Yu Zhang¹, Yanyan Li², Yanmin Shan³

¹Key Laboratory of Biohazard Monitoring, Green Prevention and Control for Artificial Grassland, Ministry of Agriculture and Rural Affairs, Institute of Grassland Research of Chinese Academy of Agricultural Sciences, Hohhot, 010010, P. R. China.

Scientific data

|May 26, 2025

概括

甜菜网虫的基因组被测序,提供了染色体水平的组件来研究它的遗传学和进化. 这项研究使人们能够更深入地了解这一重要的农业害虫.

科学领域:

基因组学就是基因组学.
昆虫学昆虫学是一门学科.
农业科学农业科学

背景情况:

甜菜网虫 (Loxostege sticticalis) 是一个重要的农业害虫,但它的基因组仍然未被测序.
缺少基因组数据阻碍了对其生物学和种群遗传学的研究.

研究的目的:

测序和组装甜菜网虫 (Loxostege sticticalis) 的染色体级基因组.
为未来关于L. sticticalis.遗传学,进化和害虫管理的研究提供基础资源.

主要方法:

使用Illumina Novaseq.进行基因组测序. 6000和PacBio Sequel II平台上使用.
染色体构造捕获 (Hi-C) 用于染色体水平组装.
标准化通用单副本正义医生 (BUSCO) 进行质量评估.

主要成果:

产生了485.9 Mb的高质量的基因组组,具有很高的连续性 (连续N50: 16.4 Mb) 和支架完整性 (支架N50: 16.6 Mb).
该组件涵盖31个染色体,具有41.71%的重复含量和15913个已识别的蛋白质编码基因.
与相关的Lepidoptera物种相比,观察到高染色体合成率.

结论:

在L. sticticalis的染色体级基因组组合是Lepidoptera研究的重大进步.
这种基因组资源将促进对害虫生物学,进化和新型控制策略的研究.
这项研究增强了我们对昆虫基因组演化的理解,并为类类类中比较基因组学提供了基础.

更多相关视频

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Published on: December 28, 2016

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points

Published on: May 29, 2020

相关概念视频

Lampbrush Chromosomes

Lampbrush Chromosomes

In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops...