将基因组测序方法进行比较,以重建爱尔兰游泳池心肌炎病毒的传播情况
Andrew J Tighe1,2,3, Jens Carlsson2, Neil M Ruane1
1Marine Institute, Oranmore, Co. Galway, Ireland.
Journal of fish diseases
|May 27, 2025
概括
鱼类心肌炎病毒 (PMCV) 的遗传多样性在很大程度上位于其编码区域之外. 虽然MinION测序对于识别是准确的,但它不足以跟踪大西洋鱼的PMCV传播途径.
科学领域:
- 病毒学 病毒学
- 水产养殖是水产养殖的一种方式.
- 基因组学就是基因组学.
背景情况:
- 游泳池肌心炎病毒 (PMCV) 在大西洋鱼中引起心肌病综合征 (CMS).
- 在爱尔兰,PMCV对鱼养殖业构成重大疾病威胁.
- 了解PMCV传播对于疾病管理至关重要.
研究的目的:
- 分析爱尔兰PMCV菌株的整个基因组多样性.
- 评估MinION测序对PMCV诊断和传播研究的有用性.
- 为了调查PMCV菌株在爱尔兰的起源.
主要方法:
- 爱尔兰PMCV菌株的全基因组测序.
- 对于MinION和Illumina MiSeq测序平台进行比较.
- 来自爱尔兰和法罗群岛的PMCV序列的家族遗传学分析.
主要成果:
- 超过80%的PMCV遗传多样性位于常用测序的开放阅读框架 (ORF) 外.
- MinION测序实现了与Illumina MiSeq99.59%的相同性,足以识别病原体,但无法追踪传播.
- 与法罗群岛菌株的比较表明,一些爱尔兰PMCV菌株可能起源于野生鱼类.
结论:
- PMCV的遗传多样性广泛,位于非编码区域.
- MinION测序为PMCV识别提供了一个可行的选择,但需要更高的精度来分析传播途径.
- 野生鱼群可能是PMCV引入养殖鱼的来源.
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