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AM-DMF-ddRPA:一个全集的数字微流体平台,用于快速和自动的数字核酸分析

Jiajian Ji^1,2, Xinpei Pang^1,2, Chunyu Chang²

¹School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230026, P.R. China.

Angewandte Chemie (International ed. in English)

|May 27, 2025

概括

一种新的自动化微流体学方法在不到一个小时内迅速量化流感A和B病毒 (IAV和IBV). 这种数字核酸量化分析为疾病诊断提供了高灵敏度.

科学领域:

生物技术是生物技术.
微流体学微流体学
分子诊断学分子诊断学

背景情况:

准确的核酸量化分析 (NQA) 对疾病管理至关重要.
当前的数字NQA方法往往缺乏自动化和速度.
为了及时诊断,需要快速和自动化的NQA.

研究的目的:

为核酸量化开发一种快速和自动化的数字微流体学方法.
为了有效量化流感A和B病毒 (IAV和IBV).
为了实现高通量,用于疾病诊断的单分子检测.

主要方法:

开发了一种基于活性矩阵数字微流体的滴滴数字重组聚合酶放大 (AM-DMF-ddRPA) 方法.
制造了一个AM-DMF芯片,配有高通量电极和人工智能滴滴导航算法.
实施了一种双目标RPA系统,用于同时检测IAV和IBV.

主要成果:

在60分钟内实现了从核酸提取到分析的完整工作流自动化.
通过使用人工智能驱动的导航成功操纵了4608滴 (每滴0.9nL).
证明了超高灵敏度,每次反应检测极限为1.69个副本.
报告的100%一致性与使用临床样本的定量PCR (qPCR) 结果.

结论:

AM-DMF-ddRPA方法为NQA提供了一个快速,自动化和高度敏感的解决方案.
这项技术显著推进了疾病诊断,治疗监测和研究.
该平台显示出在分子诊断中广泛应用的巨大潜力.

关键词:

人工智能的人工智能是人工智能.数字微流体 (DMF) 是指数字微流体.滴滴数字重组酶聚合酶放大 (ddRPA) 技术在芯片上的实验室 (LOC)微电子设备微电子设备病毒检测病毒检测

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