作为定量质谱成像方法的标准加法.
Lucie Davidová1, Ingela Lanekoff1,2
1Department of Chemistry─BMC, Uppsala University, Box 576, 751 23 Uppsala, Sweden.
Analytical chemistry
|May 27, 2025
概括
在质谱成像 (MSI) 中量化分析物具有挑战性. 这项研究引入了一种使用组织提取物进行具有成本效益和多功能性的像素对像素量化的新型标准添加方法,克服了内部标准的局限性.
科学领域:
- 分析化学 分析化学
- 生物医学成像技术 生物医学成像技术
背景情况:
- 由于复杂的样本矩阵,质谱成像 (MSI) 中的量化很困难.
- 同位素标记的内部标准 (IS) 通常用于相对量化,但具有预选需求和成本等局限性.
- 需要使用替代方法来准确和可访问的MSI量化.
研究的目的:
- 开发和验证一种新的MSI量化工作流程,使用标准加法.
- 将标准加法与现有方法 (如IS量化和外部校准) 的性能进行比较.
- 证明使用内源性组织分子作为MSI标准添加标准校准标准的可行性.
主要方法:
- 开发了一种工作流程,用于采集和处理使用MSI中的标准加法方法的定量数据.
- 应用MSI实验的标准补充,使用组织提取物作为校准标准.
- 对比标准加法与IS量化和外部校准方法的量化结果.
主要成果:
- 标准加法为MSI中的像素对像素量化提供了一种可行的方法.
- 通过标准加法获得的量化结果与IS量化和外部校准的结果进行了比较.
- 证明了内源性组织分子的成功应用,作为标准添加量化标准的容易访问的标准.
结论:
- 拟议的标准加法为MSI量化提供了一个具有成本效益,可访问性和多功能性的替代方案.
- 这种方法克服了与传统方法相关的局限性,例如同位素标记IS的成本和可用性.
- 使用内源分析物作为校准标准提高了MSI中标准添加的实用性和适用性.
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