Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

544
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
544
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

632
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
632
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

784
When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
784

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

On-Chip Raman Spectroscopy for Rapid Antimicrobial Susceptibility Testing from Blood Cultures.

Analytical chemistry·2026
Same author

From bulk samples to single cells: measurement strategies in Raman-based antibiotic susceptibility testing.

Analytical and bioanalytical chemistry·2026
Same author

Current trends in machine learning for surface-enhanced Raman spectroscopy.

The Analyst·2026
Same author

Lightweight CycleGAN models for cross-modality image transformation and experimental quality assessment in fluorescence microscopy.

Biomedical optics express·2026
Same author

Complex-Valued Chemometrics for Analyzing Absorbance or Raman Spectra.

Analytical chemistry·2026
Same author

Environmental sustainability assessment of reverse-phase liquid chromatography for retention and ionization profiling of angiotensin-converting enzyme inhibitors.

Journal of pharmaceutical and biomedical analysis·2026
Same journal

A modular microfluidic chip unit for multiple-organ-on-chip platform integration and drug screening.

The Analyst·2026
Same journal

Outstanding Reviewers for <i>Analyst</i> in 2025.

The Analyst·2026
Same journal

From silver nanoparticles to nanoclusters: enhanced oxygen evolution electrocatalysis through size reduction.

The Analyst·2026
Same journal

Advances in application of microplasmas for non-metallic species analysis by optical spectrometry.

The Analyst·2026
Same journal

Sulfur vacancy-mediated self-photocatalysis-boosted electrochemiluminescence sensing <i>via</i> molecular oxygen activation for sensitive detection of isocarbophos.

The Analyst·2026
Same journal

Analytical challenges in mapping the subcellular metabolome and lipidome.

The Analyst·2026
查看所有相关文章

相关实验视频

Updated: Sep 20, 2025

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional &#960;-conjugate Systems
09:57

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

7.3K

拉曼光谱仪的长期设备稳定性

Shuxia Guo1,2, Anuradha Ramoji1,2, Aikaterini Pistiki1,2

  • 1Leibniz Institute of Photonic Technology, Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Albert Einstein Strasse 9, 07745 Jena, Germany. shuxia.guo@uni-jena.de.

The Analyst
|May 27, 2025
PubMed
概括
此摘要是机器生成的。

长期拉曼光谱仪器的稳定性在10个月内使用13种参考物质进行了评估. 计算方法,包括变化自编码器 (VAE) 和广泛的乘法散射校正 (EMSC),显著减少了光谱变化,提高了数据可靠性.

更多相关视频

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
15:04

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Published on: May 18, 2011

13.2K
Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

17.2K

相关实验视频

Last Updated: Sep 20, 2025

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional &#960;-conjugate Systems
09:57

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

7.3K
Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
15:04

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Published on: May 18, 2011

13.2K
Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

17.2K

科学领域:

  • 分析化学 分析化学
  • 频谱学是一种光谱学.
  • 工具分析 工具分析

背景情况:

  • 长期稳定性对于可靠的拉曼光谱应用至关重要,特别是在疾病诊断中.
  • 设备漂移可以损害数据的准确性,并导致重大后果.
  • 需要进行系统的调查,以了解和减轻仪器变化.

研究的目的:

  • 系统地研究拉曼光谱仪器的长期仪器稳定性.
  • 在10个月的时间内量化设备相关的变化.
  • 探索用于减少光谱变量的计算方法.

主要方法:

  • 在10个月内每周对13种不同的质量控制物质进行测量.
  • 获得每种物质每次测量每天大约50个拉曼光谱.
  • 开发一个数据管道来分析强度变化,相关系数,聚类和稳定性基准测试的分类.

主要成果:

  • 发现拉曼装置的变化主要是随机的,而不是系统的.
  • 计算方法,特别是变化自编码器 (VAE) 网络和广泛的乘法散射校正 (EMSC),在减少光谱变化方面是有效的.
  • 在独立测量日中,对分类任务的预测准确度得到了改进.

结论:

  • 长期的拉曼装置稳定性可以通过计算数据处理显著提高.
  • VAE和EMSC的组合提供了一种有希望的方法来缓解光谱变化.
  • 通过稳定性校正提高数据可靠性,支持拉曼光谱在关键领域的更广泛应用.