Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Transcription01:10

Transcription

Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Transcription01:17

Transcription

Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Transforming Growth Factor beta-2 (TGFβ2) Drives Trabecular Meshwork Progenitor Cell Differentiation Through SMAD2/3 Signalling.

Stem cells (Dayton, Ohio)·2026
Same author

Monogenic and Polygenic Risk for Kidney Cancer in Two Large Biobanks.

European urology oncology·2026
Same author

HSPA4 and SYVN1 positivity in osteoarthritis synovium as indicators of proteostasis dysfunction.

Clinical and experimental rheumatology·2026
Same author

A probabilistic risk framework for microplastics integrating uncertainty across toxicological and environmental variability: Development and application to marine and freshwater ecosystems.

Journal of hazardous materials·2026
Same author

MicroRNA-29b targets ADAM12 and 19 to regulate the extracellular matrix in lamina cribrosa cells.

Cell and tissue research·2025
Same author

Gene Expression in Muscle-Invasive and Non-Muscle-Invasive Bladder Cancer Cells Exposed to Hypoxia.

Cancers·2025

相关实验视频

Updated: Jun 30, 2026

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes
08:23

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

Published on: December 28, 2011

17.5K

脊椎网状网状细胞原始细胞的转录组概况.

Xiaochen Fan1, Stephanie Kennedy1, Emine K Bilir1

  • 1Department of Eye and Vision Science, Institute of Life Course and Medical Sciences, University of Liverpool, Liverpool, United Kingdom.

Stem cell reviews and reports
|May 27, 2025
PubMed
概括
此摘要是机器生成的。

椎间板状网状前代细胞 (TMPCs) 具有独特的基因表达特征,与其他TM细胞不同. 这项研究确定了新型标记物和途径,这些标记物和途径对于光眼研究中TMPC功能至关重要.

关键词:
细胞分化的过程主要的开放角光眼.原始细胞是原始细胞.在RNA-Seqq.椎间板状网状网状网 椎间板状网状网状网状网

更多相关视频

Single-cell Profiling of Developing and Mature Retinal Neurons
10:20

Single-cell Profiling of Developing and Mature Retinal Neurons

Published on: April 19, 2012

14.3K
Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis
13:47

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis

Published on: June 3, 2018

9.4K

相关实验视频

Last Updated: Jun 30, 2026

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes
08:23

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

Published on: December 28, 2011

17.5K
Single-cell Profiling of Developing and Mature Retinal Neurons
10:20

Single-cell Profiling of Developing and Mature Retinal Neurons

Published on: April 19, 2012

14.3K
Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis
13:47

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis

Published on: June 3, 2018

9.4K

科学领域:

  • 眼科医生 眼科 眼科
  • 细胞生物学 细胞生物学
  • 基因组学就是基因组学.

背景情况:

  • 脊椎网状 (TM) 细胞的损失和功能障碍与衰老和初级开角玻璃眼有关.
  • 肌痛转移前体细胞 (TMPCs) 的身份和对肌痛转移健康的贡献仍然不清楚.

研究的目的:

  • 为了确定人类TM细胞 (TMPCs) 的基因表达特征.
  • 阐明TMPC行为所涉及的独特标记物和途径.

主要方法:

  • 主要TM细胞 (PTM),非分化的球体 (TMPC) 和重新分化的TM细胞 (DTM) 的RNA测序 (RNA-Seq).
  • 生物信息学分析使用Tuxedo,Bowtie2,Tophat,袖口链条和IPA.
  • 通过纳米链,RT-qPCR,免疫细胞化学和西部涂抹验证.

主要成果:

  • 与PTM和DTM细胞相比,RNA-Seq在TMPC中发现了显著的差异表达基因 (DEG).
  • 在TMPC中增加了70个DEGs;在TMPC中确定了SOX2和NOTCH1等独特标记物.
  • 途径分析显示TMPCs中细胞周期,SUMOylation和STAT3途径的激活.

结论:

  • 这项研究揭示了TMPCs的新型基因表达特征,突出了独特的标记物和激活的途径.
  • 研究结果为TMPC行为提供了关键的见解,这些见解与TM生理学和玻璃眼病原体相关.