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相关概念视频

Fluorescence and Phosphorescence: Instrumentation01:25

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Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
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相关实验视频

Updated: May 4, 2026

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
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光终身成像显微镜的实用指南

Nadia Sarfraz1, Esther Braselmann1

  • 1Department of Chemistry, Georgetown University, Washington, DC 20057.

Molecular biology of the cell
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PubMed
概括
此摘要是机器生成的。

光终身成像显微镜 (FLIM) 通过感知分子环境并实现RNA量化,提供先进的细胞分析. 本指南使研究人员能够利用FLIM用于各种生物传感器和显微镜应用.

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Fluorescence Lifetime Macro Imager for Biomedical Applications
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相关实验视频

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科学领域:

  • 生物光子学和先进的显微镜技术.

背景情况:

  • 光终身成像显微镜 (FLIM) 与传统的基于强度的方法相比,提供了独特的优势,包括对分子环境的敏感性和度独立性.
  • 最近,FLIM已经适用于活细胞中的定量RNA传感,从而创建了Riboglow-FLIM平台.
  • 该技术具有很大的潜力,可以补充和增强现有的显微镜平台.

研究的目的:

  • 为使用FLIM与各种光传感器提供实用指南.
  • 为研究人员介绍设计和执行FLIM实验的基本概念和最佳实践.
  • 通过现实世界的案例研究来证明FLIM工作流程的广泛适用性.

主要方法:

  • 讨论FLIM基础知识,包括操作原则和数据采集.
  • 对FLIM的实验设置考虑的解释.
  • 关于 FLIM 数据相关的数据拟合原则和分析技术的概述.

主要成果:

  • 使用Riboglow-FLIM平台,展示FLIM在活细胞中的定量RNA传感能力.
  • 案例研究展示了FLIM与具有良好的特征光蛋白的应用.
  • 验证各种光传感应用的FLIM工作流程.

结论:

  • FLIM是一种强大的方式来感知分子环境,结合事件和新陈代谢.
  • 里博格洛-FLIM平台代表了活细胞定量RNA分析的重大进步.
  • 本指南为研究人员提供了有效实施FLIM用于各种生物传感应用的知识.