Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

2.8K
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
2.8K
Small GTPases - Ras and Rho01:24

Small GTPases - Ras and Rho

4.3K
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
4.3K
The Ras Gene02:38

The Ras Gene

6.5K
The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
6.5K
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

4.9K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
4.9K
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

2.8K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
2.8K
Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

2.4K
Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
2.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Defining rapid early progression in glioblastoma-A volumetric approach.

Neuro-oncology advances·2026
Same author

Assessment of real-world surveillance strategies for patients undergoing systemic therapy for brain metastases.

Frontiers in oncology·2026
Same author

Surface-engineered dual drug-loaded tumor-targeted liposomal nanoparticles to overcome the therapeutic resistance in glioblastoma multiforme.

Communications medicine·2026
Same author

The Extracellular Matrix Regulates Invasion in Fusion-Negative Rhabdomyosarcoma via YAP-PIEZO1 Signaling Axis.

Cancers·2026
Same author

Outcomes for Sinonasal Undifferentiated Carcinoma (SNUC): An International Multi-Center Retrospective Cohort Study.

Cancers·2026
Same author

Spontaneous Resolution of 2 High Flow Cervical Vertebral Arteriovenous Fistulas.

Stroke (Hoboken, N.J.)·2026

相关实验视频

Updated: Sep 20, 2025

Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs
10:32

Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs

Published on: December 19, 2019

11.1K

YAP通过Rho GTPase开关控制细胞迁移和入侵.

Sagar R Shah1,2, Chunxiao Ren3, Nathaniel D Tippens2

  • 1Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA.

Science signaling
|May 27, 2025
PubMed
概括

转录协活性剂YAP通过激活TRIO促进细胞迁移,从而导致Rac1激活和RhoA抑制. 这种YAP-TRIO-Rho信号通路对于细胞入侵至关重要,特别是在质母细胞瘤中.

更多相关视频

All-optical Mechanobiology Interrogation of Yes-associated Protein in Human Cancer and Normal Cells using a Multi-functional System
09:55

All-optical Mechanobiology Interrogation of Yes-associated Protein in Human Cancer and Normal Cells using a Multi-functional System

Published on: December 20, 2021

3.1K
Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

7.0K

相关实验视频

Last Updated: Sep 20, 2025

Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs
10:32

Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs

Published on: December 19, 2019

11.1K
All-optical Mechanobiology Interrogation of Yes-associated Protein in Human Cancer and Normal Cells using a Multi-functional System
09:55

All-optical Mechanobiology Interrogation of Yes-associated Protein in Human Cancer and Normal Cells using a Multi-functional System

Published on: December 20, 2021

3.1K
Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

7.0K

科学领域:

  • 细胞生物学 细胞生物学
  • 分子瘤学分子瘤学
  • 癌症研究 癌症研究

背景情况:

  • 细胞运动对生理和病理过程至关重要.
  • 转录协活性剂YAP在发育中起作用,并与癌症转移有关.
  • 罗家族GTPases (Rac1,RhoA) 是细胞骨动力学和细胞运动性的关键调节者.

研究的目的:

  • 阐明YAP控制细胞迁移的机制.
  • 研究YAP在调节Rho GTPase信号传递中的作用.
  • 探索针对癌症,特别是质母细胞瘤中YAP途径的治疗潜力.

主要方法:

  • 研究了YAP通过内部增强剂结合对TRIO的转录调节.
  • 评估了YAP-TRIO信号对Rac1和RhoA激活的影响.
  • 在体外和体内生物中评估细胞迁移和侵入.
  • 在患者质母细胞瘤样本中分析了基因表达特征 (YAP,TRIO,STAT3).

主要成果:

  • 雅普直接对Rac1关氨酸核酸交换因子TRIO进行了交换.
  • 由YAP诱导的TRIO激活导致Rac1激活和RhoA抑制,增强细胞迁移和入侵.
  • 这种依赖于YAP的途径在各种细胞类型中被观察到,在质母细胞瘤中尤其明显.
  • YAP-TRIO信号激活了STAT3,这表明路径交叉交谈可能会促进侵入性生长.

结论:

  • YAP-TRIO-Rho-GTPase信号网络是侵入性细胞扩散的关键调节器.
  • YAP,TRIO和STAT3的过活化与质母细胞瘤患者的生存率差有关.
  • 针对这种途径可能为侵袭性癌症提供治疗策略.