普雷沃特拉中间体协同加剧口腔链球菌诱导的肺炎
Hiroki Ashizawa1,2, Naoki Iwanaga2, Kazuki Nemoto3
1Department of Respiratory Medicine, Graduate School of Biomedical Sciences, Nagasaki University.
普雷沃特拉介质通过破坏中性粒细胞功能,恶化口腔链球菌种类引起的肺炎. 在P.intermedia中,第九类分泌系统对这种恶化至关重要,提供了潜在的治疗点.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 肺部病理学 肺部病理学
背景情况:
- 口腔无氧细菌引起的呼吸道感染的机制尚未完全理解.
- 在社区获得的肺炎可能涉及口腔细菌的隐藏混合感染.
- 需要阐明Prevotella intermedia在恶化链球菌引起的肺炎中的作用.
研究的目的:
- 调查Prevotella intermedia (P. intermedia) 如何恶化口腔链球菌种所引起的肺炎.
- 确定这种恶化所涉及的特定机制和毒性因素.
主要方法:
- 在小鼠中,口腔链球菌与P.intermedia培养超 (PiSup) 进行了口腔管治疗.
- 评估了生存率,微生物负担和炎症反应.
- 大量RNA测序分析了宿主反应,基因工程P.中间菌株确定了致病因素.
主要成果:
- 结合性链球菌 spp. 的结合性链球菌. 和PiSup显著降低了小鼠的生存率,增加了肺/脏微生物负担.
- RNA测序揭示了通过NADPH下调和减少骨髓氧化酶的中性友菌杀菌活性中断.
- 在P.intermedia的第九类分泌途径 (ΔporT或ΔporK) 的突变阻止了混合感染的恶化.
结论:
- 在混合感染期间,P.intermedia通过引起中性友好性功能障碍而加剧链球菌引起的肺炎.
- 第九类分泌系统产品是关键的毒性因素和潜在的治疗点,独立于以前已知的因素.
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