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直接对PCR (D2P) 方法的评估,用于传染病的分子诊断

Rahul Sharma1, Vaibhav K Tamrakar2, Rob E Carpenter3

  • 1Department of Research, Scienetix, Tyler, TX 75703, USA; Department of Research, RetroBiotech and Research Pvt. Ltd, Jaipur, RJ, 302017, India.

Experimental and molecular pathology
|May 27, 2025
PubMed
概括

直接对PCR (D2P) 方法为诊断感染提供了一个快速,无提取的替代方法. 它的性能与传统方法相美,缩短了处理时间,提高了病原体检测的可访问性.

关键词:
临床感染 临床感染直接进入PCR.没有提取的方法.传染病诊断 传染病诊断 传染病诊断分子诊断学 分子诊断学核酸提取方法 核酸提取方法病原体检测检测 病原体检测

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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