在Strongylocentrotus intermedius中由Vibrio alginolyticus诱导的细胞外陷形成的信号通路
Haoyu Zhang1, Wei Chen2, Xiaochen Zhang1
1School of fisheries, Ludong University, Yantai, Shandong 264025, China.
概括
海使用细胞外陷 (ETs) 来获得对细菌的天生的免疫力. 线粒体和糖解是ET形成的关键,涉及特定的基因和细胞防御的免疫路径.
科学领域:
- 海洋生物学 海洋生物学
- 免疫学 免疫学 免疫学
- 无脊椎动物动物学 不脊椎动物动物学
背景情况:
- 细胞外陷 (ETs) 是许多物种中至关重要的先天免疫成分.
- 海,Strongylocentrotus intermedius,完全依赖天生的免疫力来抵抗疾病.
研究的目的:
- 研究由Vibrio alginolyticus刺激的S. intermedius大胆细胞中ET的形成和机制.
- 确定线粒体,糖解和特定基因在ET生产中的作用.
主要方法:
- 用V. alginolyticus在不同度中刺激胆红细胞.
- 流细胞测量用于分析ET形成期间的线粒体指标.
- 转录组分析以识别差异表达基因 (DEG) 并构建蛋白质-蛋白质相互作用 (PPI) 网络.
- 定量实时PCR (qRT-PCR) 用于基因表达的验证.
主要成果:
- 增加的V. alginolyticus度导致更显著的ET形成.
- ET形成与线粒体指标的变化相关,并涉及糖解和ATP合成.
- 转录组分析确定了2631个DEG,其中包括ATP6,ND2,G3PDH和MAPK7等枢纽基因,与线粒体和免疫通路有关.
- ET形成调节了包括TNF,NF-κB,MAPK,PI3K-AKT和mTOR在内的关键免疫调节剂.
结论:
- 在S. intermedius中由V. alginolyticus诱导的ET涉及线粒体活动和糖解.
- 该研究阐明了ET形成的分子机制及其在海中的免疫调节作用.
- 这些发现有助于理解无脊椎动物的先天免疫力,并支持S. intermedius水产养殖.
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