3年环境监测发现多种肠道病毒,包括中国东部的A76,A89,A90和C113类型
Xiaojuan Lin1, Mingyi Xu2, Yonglin Han3
1Shandong Center for Disease Control and Prevention, Jinan, 250014, China.
Food and environmental virology
|May 27, 2025
概括
使用下一代测序 (NGS) 的环境监测揭示了中国废水中的各种肠道病毒,包括新型病毒. 与传统细胞培养相比,这种方法为监测新兴肠道病毒菌株提供了优越的数据输出.
科学领域:
- 环境微生物学环境微生物学
- 病毒学 病毒学
- 分子流行病学分子流行病学
背景情况:
- 肠道病毒是一种多样化的病毒群,由于流行潜力较低,对新型病毒的全球数据有限.
- 传统的肠道病毒检测和类型的方法在捕获病毒多样性的全谱方面存在局限性.
研究的目的:
- 通过环境监测,研究新型肠道病毒的多样性和系系.
- 评估下一代测序 (NGS) 在废水中检测和表征肠道病毒的有效性.
主要方法:
- 从中国两个城市 (2019-2021) 收集了66个废水样本.
- 集中样本和进行细胞培养以隔离肠道病毒.
- 从污水缩物中提取RNA,通过RT-PCR放大VP1基因,并进行NGS.
- 对部分VP1序列进行了基因分析.
主要成果:
- 通过细胞培养检测出388种肠道病毒 (14种血清型),包括脊髓灰质炎病毒.
- NGS确定了33种肠道病毒血清型,其中埃科病毒11,考克萨基病毒A10,埃科病毒18,考克萨基病毒B4和考克萨基病毒B5是最常见的.
- 检测到新的类型 肠道病毒 A76,A89,A90 和 C113; 遗传学分析显示了不同的本地血统.
- NGS提供的数据输出远远超过了细胞培养.
结论:
- 废水含有相当多的肠道病毒,包括新发现的类型.
- 基于NGS的污水监测是检测和监测新兴肠道病毒菌株的强大工具.
- 这种方法比传统的细胞培养为综合性肠道病毒监测提供了优势.
关键词:
检测 检测 检测 检测 检测肠道病毒 (enterovirus) 是一种病毒.下一代测序测序是什么人类的家谱 (Phylogeny) 是一个学科.类型 类型 类型 类型废水是废水,废水就是废水.更多相关视频
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