在莱迪格细胞中,诱导出外体分泌和细胞通路的破坏
Waseem Ali1,2,3, Atique Ahmed Behan4, Yonggang Ma1,2,3
1College of Veterinary Medicine, Yangzhou University, Yangzhou, China.
Journal of molecular histology
|May 27, 2025
概括
暴露会损害男性生殖雷迪格细胞,破坏外体分泌和类固醇生成. 这项研究揭示了.
科学领域:
- 生殖生物学 生殖生物学
- 毒理学 毒理学 毒理学
- 细胞生物学 细胞生物学
背景情况:
- 是一种有毒的重金属,已知会导致男性生殖系统受损.
- 对莱迪格细胞内外体分泌和细胞通路的特定影响仍然基本未知.
研究的目的:
- 研究暴露对老鼠莱迪格细胞外体分泌,类固醇生成,自和亡的影响.
主要方法:
- 在体内研究使用C57BL/6雄性小鼠暴露在化 (CdCl2) 或净化水中三个月.
- 在体外分析包括免疫组织化学,免疫光和传输电子显微镜.
- 西方斑分析用于评估与类固醇发生,自和亡相关的蛋白质表达.
主要成果:
- 暴露减少了外体分泌 (CD63,多胞体) 并破坏了莱迪格细胞形态.
- 显著降低了关键的类固醇蛋白质 (STAR,CYP11A1,CYP17A1,3BHSD1,17BHSD1,AR) 的下调.
- 通过改变相关蛋白质 (ATG5,ATG7,LC3,P62,LAMP2,Caspase-3,Caspase-8,Bax,Bcl-2) 的表达,破坏了自流并诱导了亡.
结论:
- 暴露于会对莱迪格细胞的分泌通路产生不利影响,包括外体-多胞体体内分泌.
- 会破坏细胞过程,如莱迪格细胞的自和亡.
- 这些干扰对男性生育能力构成重大风险.
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