相关实验视频

Updated: Sep 20, 2025

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

Published on: June 13, 2016

外体微RNA作为子宫内膜异位症的表观遗传生物标志物:系统性审查和生物信息学分析

Cristina Maria de Araújo Medeiros Santos¹, Amaxsell Thiago Barros de Souza², Antonia Pereira Rosa Neta³

¹Postgraduate Program in Technological Development and Innovation in Medicines, Federal University of Rio Grande do Norte, Natal 59012-570, Brazil.

International journal of molecular sciences

|May 28, 2025

概括

外体微RNA显示出作为子宫内膜异位症诊断生物标志物的潜力. 血清和组织中的差异表达突出了涉及疾病途径的特定微RNA,需要进一步验证.

科学领域:

生物标志物生物标志物
基因组学就是基因组学.
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

宫内膜异位症的诊断缺乏非侵入性生物标志物.
外体微RNA (miRNA) 是新兴的表观遗传标记物.
对子宫内膜异位症的发病因子进行外体miRNA的研究至关重要.

研究的目的:

系统地审查对外体微RNA作为子宫内膜异位症诊断生物标志物的病例控制研究.
为了确定特定的外体微RNA和涉及子宫内膜异位症发病的相关途径.

主要方法:

在主要数据库 (PubMed,Embase,Web of Science,Scopus) 进行系统的文献搜索.
包括12项病例控制研究,涉及191名子宫内膜异位症患者和169名对照.
使用纽卡斯尔-太华尺度进行质量评估.

主要成果:

在患者和对照组之间,668个外体微RNA的表达方式不同.
在血清和组织样本中经常发现特定的microRNAs (例如miR-22-3p,miR-320a,miR-200c-3p).
生物信息分析将这些微RNA与关键信号通路 (PI3K/Akt,MAPK,TGF-β) 联系起来.

结论:

外体miRNAs是对子宫内膜异位症的有希望的诊断生物标志物.
在不同的队列中进行标准化和验证是必不可少的.
未来的研究应该专注于早期诊断的临床相关性.

关键词:

生物标志物生物标志物子宫内膜异位症是什么?子宫内膜异位症是什么外基因组是外基因组的组成部分.细胞外囊泡中的细胞外囊泡.微RNAs 是一个微型RNA.

更多相关视频

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

Published on: January 7, 2019

Establishment of an Experimental Mouse Model of Endometrioma to Study its Related Infertility

Establishment of an Experimental Mouse Model of Endometrioma to Study its Related Infertility

Published on: April 5, 2024

相关概念视频

Overview of Exosomes

Overview of Exosomes

Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...