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目前用于结膜液微生物群表征的方法:系统性审查
Elena Bardi1, Daniele D'Arrigo1, Chiara Pozzi1
1IRCCS Humanitas Research Hospital, Via Manzoni 56, Rozzano, 20089 Milan, Italy.
International journal of molecular sciences
|May 28, 2025
概括
肠-关节轴将肠道微生物与关节健康联系起来. 这次审查发现突液中的细菌DNA,但不一致的方法阻碍了关节炎研究和治疗开发.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 类风湿病学 类风湿病学
背景情况:
- 肠-关节轴突出了肠道微生物群对关节健康的影响.
- 微生物越来越多地与突关节内的关节炎病原发生有关.
- 同胞液在历史上被认为是无菌的,但最近的证据表明微生物存在.
研究的目的:
- 系统地审查用于表征人类突液 (SF) 微生物组的方法.
- 在SF微生物群分析中识别当前技术及其局限性.
- 强调需要在SF微生物组研究中采用标准化的方法.
主要方法:
- 在PubMed,Embase和Web of Science的系统文献搜索,截至2025年2月5日.
- 包括的研究重点是利用下一代测序或聚合酶连锁反应来表征SF微生物组.
- 对九项研究的分析,这些研究调查了人类SF中微生物的存在.
主要成果:
- 在九项审查的研究中,有八项检测到人类突液中的细菌DNA.
- 在研究中的方法学中观察到显著的异质性.
- 样本采集,制备,污染控制,DNA提取,放大,测序和生物信息处理的不完整报告是常见的.
结论:
- 目前用于结膜液微生物组表征的方法缺乏标准化,限制了可比性和可重现性.
- 在关节炎研究中,标准化协议对于准确的SF微生物群分析至关重要.
- 改进的方法将提高对关节炎机制的理解,并为有针对性的治疗策略提供信息.
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