相关实验视频

Updated: Aug 10, 2026

Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids

Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids

Published on: March 17, 2016

感染马 herpesvirus 8 的子脏细胞的转录基因和蛋白质基因分析

Yanfei Ji¹, Dandan Xu¹, Wenxuan Si¹

¹Department of Veterinary Medicine, School of Agriculture and Biology, Liaocheng University, Liaocheng 252000, China.

|May 28, 2025

概括

在子细胞中感染马性疹病毒8 (EHV-8) 会激活关键的炎症通路,特别是TNF和NF-κB信号传递. 需要进一步的体内研究来了解马类病原体.

科学领域:

病毒学病毒学
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

马类疹病毒8 (EHV-8) 是马类动物的一个重要病原体.
了解宿主细胞对EHV-8的反应对于开发有效的对策至关重要.

研究的目的:

在子脏 (RK-13) 细胞中研究宿主细胞对EHV-8感染的反应.
确定参与宿主-病原体相互作用的关键分子通路.

主要方法:

在感染后24小时和48小时对RK-13细胞进行了转录和蛋白质组分析.
鉴定和量化了差异表达基因 (DEGs) 和蛋白质 (DEPs).
基因和基因组的京都百科全书 (KEGG) 途径分析被用来识别丰富的途径.
逆转录定量PCR (RT-PCR) 和并行反应监测 (PRM) 用于数据验证.

主要成果:

在感染后的24小时和48小时观察到大量的DEG和DEP,在48小时后的数量增加.
对KEGG通路的分析显示了与炎症相关的通路的丰富,特别是TNF和NF-κB信号传递.
验证证实了TNFR1,NF-κB p65和MAP3K8.8的一致表达趋势.

结论:

在RK-13细胞中感染EHV-8会触发宿主免疫反应,主要是通过激活TNF信号通路.
RK-13细胞可能无法完全复制马类病毒与宿主相互作用.
建议对马和驴进行体内研究,以全面了解EHV-8的病变发生.

关键词:

这种病毒是EHV-8的.细胞中的RK-13细胞.在TNF信号通路上.免疫反应的免疫反应.蛋白质组学蛋白质组学翻译学翻译学翻译学翻译学

更多相关视频

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

Published on: December 21, 2019

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

相关概念视频

Herpes

Herpes

Herpes simplex type 1 (HSV‑1) is a widespread pathogen responsible for orolabial lesions. It is an enveloped, double-stranded DNA (dsDNA) virus belonging to the family Herpesviridae. Once the virus infects a host cell, its double‑stranded DNA genome is delivered into the nucleus, where a coordinated cascade of immediate‑early, early, and late gene expression directs viral DNA replication, structural protein synthesis, and virion assembly. After primary infection of epithelial cells, HSV-1...