甲基-微-C:同时描述染色质可访问性,相互作用和DNA甲基化
Leonardo Gonzalez-Smith1, Claire Stevens1, Huan Cao1
1Department of Cancer Biology and the Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, CA 90089, United States.
NAR genomics and bioinformatics
|May 28, 2025
概括
研究人员开发了Methyl-Micro-C (MMC),这是一种新技术,可以同时绘制DNA甲基化,染色质可访问性和染色质相互作用的地图. 这种高效的方法有助于理解表观基因组及其在发育和疾病中的作用.
科学领域:
- 基因组学和表观遗传学
- 分子生物学分子生物学
- 生物技术是生物技术.
背景情况:
- 表观基因组,包括染色质可访问性,相互作用和DNA甲基化,对于基因调节至关重要,并因细胞类型而异.
- 了解这些表观遗传特征是揭示发育和疾病机制的关键.
- 现有的方法通常只对单个表观遗传特征进行分析,从而限制了全面的分析.
研究的目的:
- 引入一种新的技术,用于从同一样本中同时分析多个表观遗传特征.
- 为了能够有效和精确地分析三维表观基因组,特别是在资源限制下.
主要方法:
- 开发了Methyl-Micro-C (MMC),一种结合MNase介导碎片化与酶转化的技术.
- 在单个样本中,MMC同时测量染色质可访问性,染色质相互作用和DNA甲基化.
- 该方法增强了染色质相互作用的分辨率和CpG覆盖率.
主要成果:
- 通过捕捉一致的染色质可访问性,染色质相互作用和DNA甲基化信号,成功地描述了三维表观基因组.
- 证明了MMC技术的效率和简单实施.
- 提供了一种用于同时测量关键表观遗传标记的方法.
结论:
- 甲基微C (MMC) 提供了一种高效且易于使用的方法,用于同时进行多个表观遗传学分析.
- 这种技术有助于更深入地了解表观基因组调节在发育和疾病中的作用.
- 对于需要从有限样本获得精确的多原子表观遗传数据的研究人员来说,MMC是一个有价值的工具.
更多相关视频
24:02The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
Published on: April 11, 2014
18.3K
10:41An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
Published on: April 5, 2018
10.5K
相关概念视频
02:36Chromatin Immunoprecipitation- ChIP
11.3K
Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
11.3K
02:25Spreading of Chromatin Modifications
8.5K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
Writers
The writer...
8.5K
03:17Inheritance of Chromatin Structures
6.6K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.6K
01:01Euchromatin
7.6K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
7.6K
02:38Heterochromatin
14.6K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
14.6K
02:32Histone Modification
14.2K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
14.2K