相关实验视频
Updated: Sep 20, 2025
09:45
Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
Published on: February 9, 2012
25.4K
使用基于分子旋转器的化探针对蛋白质进行敏感的检测和标记
Na Chen1, Qingyi Wu1, Xia Yuan1
1State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of New Drug Design, Frontiers Science Center for Materiobiology and Dynamic Chemistry, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China. xiehexin@ecust.edu.cn.
Organic & biomolecular chemistry
|May 28, 2025
概括
一个新的化探针通过增强的光在与氨基残留结合后检测和标记蛋白质. 该工具可实现实时细胞成像和快速抗生素敏感性测试.
科学领域:
- 生物化学 生化学
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 蛋白质检测和标记在生物研究中至关重要.
- 现有的方法通常需要多个步骤,或者不适合活细胞成像.
- 需要开发敏感和实时探测器.
研究的目的:
- 开发一种敏感的化试剂,用于蛋白质检测和标记.
- 为了证明其在活细胞成像和抗生素敏感性测试中的实用性.
主要方法:
- 设计和合成一个对环境敏感的分子旋转器探针.
- 探针与蛋白质氨基酸残留结合的特征与自由氨基酸的特征.
- 该探头的应用是对活细胞中的膜蛋白进行实时成像.
- 使用探针来确定抗生素的最小抑制度 (MIC).
主要成果:
- 探测器表现出对环境敏感的光,在与蛋白质结合时显著增强了排放.
- 探测器显示可逆的,与自由氨基酸的非光相互作用.
- 在活细胞中成功地实现了对膜蛋白的无洗,实时成像.
- 证明了抗生素MIC对细菌的快速确定.
结论:
- 开发的化试剂是用于蛋白质检测和标记的敏感和多功能工具.
- 它的实时,无需洗的活细胞成像能力为细胞生物学开辟了新的途径.
- 探测器在快速MIC确定中的实用性为微生物学和药物发现提供了有价值的应用.
相关概念视频
03:31Labeling DNA Probes
8.4K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.4K
01:19Protein Dynamics in Living Cells
2.3K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.3K