相关实验视频
Updated: Sep 20, 2025

08:01
The Soft Agar Colony Formation Assay
Published on: October 27, 2014
112.3K
通过增加CHEK1的表达,FOXM1促进非小细胞肺癌的进展
Xiao-Ning Lu1,2,3, Jun Chen1,2, Guang Han4
1Department of Thoracic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, 215006, China.
Current medical science
|May 28, 2025
概括
检查点激酶1 (CHEK1) 促进非小细胞肺癌 (NSCLC) 的生长和扩散. 叉头盒蛋白M1 (FOXM1) 调节CHEK1,这表明两者都是潜在的NSCLC治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
背景情况:
- 非小细胞肺癌 (NSCLC) 是癌症死亡的主要原因.
- 了解推动NSCLC进展的分子机制对于开发有效的治疗方法至关重要.
研究的目的:
- 研究检查点激酶1 (CHEK1) 在NSCLC中的作用.
- 探索NSCLC中CHEK1和分叉盒蛋白M1 (FOXM1) 之间的调节关系.
主要方法:
- 使用Transwell和CCK-8试验评估NSCLC细胞迁移,入侵和扩散.
- 分析了通过西式涂抹 (Western blotting) 分析表皮层-介质细胞过渡 (EMT) 标记物.
- 在使用实时定量PCR的NSCLC组织中量化CHEK1和FOXM1表达.
- 在动物模型中验证了CHEK1对瘤生长的影响.
- 通过使用双露西法酶报告者和ChIP测试,研究了FOXM1与CHEK1促进体的结合.
主要成果:
- 发现FOXM1和CHEK1在NSCLC组织中受到上调.
- CHEK1过度表达增强了NSCLC细胞增殖,而敲击抑制了增殖,EMT和瘤生长.
- 福克斯M1直接与CHEK1促进体结合,导致CHEK1的表达增加.
结论:
- CHEK1在促进NSCLC扩散和瘤生长方面发挥着重要作用.
- FOXM1直接调节CHEK1的表达,突出显示NSCLC的一个关键分子相互作用.
- 无论是CHEK1还是FOXM1,都代表了NSCLC治疗的有希望的治疗点.
更多相关视频
相关概念视频
mTOR Signaling and Cancer Progression
3.9K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.9K
Tumor Progression
6.5K
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
6.5K
Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
Induced Pluripotent Stem Cells
4.4K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
4.4K
PI3K/mTOR/AKT Signaling Pathway
4.0K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
4.0K
Mouse Models of Cancer Study
5.7K
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
5.7K

