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纳灵宁通过调节TLR4/NF-κB通路介导的炎症反应来抑制PRV复制

Juanjuan Xu¹, Zhiying Wang¹, Zhiyuan Ren¹

¹College of Veterinary Medicine, Northeast Agricultural University, Harbin 150038, China.

Veterinary microbiology

|May 28, 2025

概括

纳灵宁是一种黄类化合物,通过向TLR4/NF-κB通路,有效地抑制伪狂犬病病毒 (PRV) 扩散,并减少炎症. 这种天然化合物对猪的PRV治疗和预防具有重大潜力.

科学领域:

病毒学病毒学
免疫学免疫学免疫学
药理学药理学是指药理学的学科.

背景情况:

伪狂热病毒 (PRV) 在猪业中造成重大经济损失.
目前缺乏有效的治疗方法,需要开发新的抗PRV药物.
PRV感染通过TLR4/NF-κB通路触发炎症反应.

研究的目的:

识别和评估用于PRV治疗的新型化合物.
为了研究naringenin的抗PRV机制.
在小鼠模型中评估纳灵宁的治疗潜力.

主要方法:

对单体化合物的抗PRV活性进行选.
在体外测试中使用PK-15细胞来评估naringenin对PRV的抑制.
在基因和蛋白质水平上对TLR4/NF-κB通路激活的分析.
在体内研究,使用小鼠模型来评估生存率和病毒载量.

主要成果:

纳林根因在PK-15细胞中表现出强有力的,剂量依赖的PRV增殖抑制.
纳灵宁抑制了TLR4和P65蛋白质的表达,抑制了NF-κB的激活.
纳灵宁治疗减少了促炎媒介 (IL-18,IL-1β,TNF-α,IL-6) 和P65核转位.
纳灵宁显著改善了PRV感染小鼠的生存率,并减少了病毒复制.

结论:

纳灵宁表现出显著的抗PRV活性.
纳灵宁通过调节TLR4/NF-κB信号通路并减少炎症来发挥其作用.
纳灵宁代表了一种有前途的治疗策略,用于预防和控制伪狂犬病.

关键词:

在体内和体外.在Naringenin,我们可以看到.伪狂犬病病毒病毒.在 TLR4/NF-κBB 中.

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