P450双化合成酶ShyB在前体上催化了His-C2和Tyr-O4之间的交叉链接
Abujunaid Habib Khan1, Jabal Rahmat Haedar1, Vic Kiselov1
1Latvian Institute of Organic Synthesis, Aizkraukles Street 21, LV-1006 Riga, Latvia.
Organic letters
|May 28, 2025
概括
研究人员确定了435种与比亚利前体相关的P450酶. 发现ShyB酶可催化核糖体合成和翻译后修饰 (RiPPs) 中的交叉链接.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 自然产品 化学 化学
背景情况:
- 比亚利利是一种新型的核糖体合成和翻译后修饰 (RiPPs) 类.
- 这些的特点是独特的二交叉链 (C-C,C-N或C-O) 由P450二利合成酶 (P450s) 形成.
- 了解负责这些修饰的酶对于发现新的RiPPs至关重要.
研究的目的:
- 为了识别和描述参与比亚利化生物合成的新型P450酶.
- 扩大已知的双利前体和它们的相关酶的库存.
- 为了功能验证一种新发现的酶,该酶可催化RiPPs中的二甲基交叉链接.
主要方法:
- 手动识别同源的P450酶与假定的双利前体酸相关.
- 生物信息分析,以策划一个广泛的列表的biarylitide前体.
- 在大肠杆菌中进行体内功能研究,以表征酶活性.
主要成果:
- 编制了一份包括435个同源的P450s与比亚利前体相关的综合清单.
- 鉴定了来自Streptomyces clavuligerus F613-1的ShyB酶. 这种酶被认为是ShyB.
- 已经证明ShyB可催化His-C2和Tyr-O4之间在E. coli的前体ShyA上的交叉链的形成.
结论:
- 这项研究显著扩大了已知的双利生物合成基因集群的多样性.
- 在ShyB的功能性特征提供了一个酶催化RiPPs特定的双交叉链接的第一个例子.
- 这些发现为发现和设计具有独特结构特征的新型RiPP铺平了道路.
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