相关实验视频
Updated: Sep 20, 2025

13:07
A Protocol for Transcranial Photobiomodulation Therapy in Mice
Published on: November 18, 2018
12.3K
光生物调节疗法:在2型糖尿病中对胰岛素抵抗有前途的治疗方法
Jaber Zafari1, Hanieh Sadeghi2, Hossein Abbasinia2
1Department of Pharmacology and Toxicology, TeMS.C., Islamic Azad University, Tehran, Iran.
In vitro cellular & developmental biology. Animal
|May 28, 2025
概括
光生物调节疗法 (PBMT) 通过改善胰岛素耐药性来治疗2型糖尿病具有前景. 这种低功率光疗在细胞模型中降低了甘油三水平和葡萄糖吸收,而不会损害细胞活力.
科学领域:
- 生物医学工程 生物医学工程
- 代谢研究研究 代谢研究
- 细胞生物学 细胞生物学
背景情况:
- 2型糖尿病 (T2DM) 是一种普遍的代谢障碍,其特征是胰岛素抵抗 (IR).
- 葡萄糖载体4 (GLUT4) 功能受损以及脂肪和肌肉组织中异常的胰岛素信号传递是T2DM的关键因素.
- 功能障碍的脂肪组织通过增加甘油三和脂肪酸水平而加剧了IR.
研究的目的:
- 研究光生物调节疗法 (PBMT) 作为治疗胰岛素抵抗的治疗干预的潜力.
- 在细胞模型中评估PBMT对葡萄糖代谢,脂肪生成和胰岛素信号传递的关键分子调节者的影响.
主要方法:
- 利用3T3-L1预adipocytes在高葡萄糖环境中分化为adipocytes,以建立胰岛素抵抗模型.
- 通过MTT试验评估细胞活力,并通过油红色O染色量化脂质积累.
- 在PBMT后使用qRT-PCR分析葡萄糖摄取和基因表达 (FOXO1,GFAT-2,PTP1B).
主要成果:
- PBMT并没有损害前脂肪细胞或脂肪细胞的生存能力.
- 在已建立的胰岛素抵抗模型中,PBMT显著降低了甘油三水平和葡萄糖吸收.
- 与脂肪生成和胰岛素信号通路相关的PBMT调节的基因表达特征.
结论:
- PBMT证明了作为一种非侵入性治疗策略,用于管理胰岛素耐药性及其代谢后果的潜力.
- 这些发现表明,PBMT可以改善脂肪细胞功能和葡萄糖平衡,尽管需要进一步的机制研究.
相关概念视频
Insulin: Dosing Regimen and Adverse Effects
271
Insulin-replacement therapy usually includes both long-acting insulin (basal) and short-acting insulin (to cater to postprandial needs). In a diverse group of type 1 diabetes patients, the average daily insulin dose is typically 0.5-0.7 units/kg body weight. However, obese patients and pubertal adolescents may need more due to insulin resistance.
The basal dose constitutes about 40%-50% of the total daily dose, with the rest as premeal insulin. The mealtime insulin dose should mirror...
The basal dose constitutes about 40%-50% of the total daily dose, with the rest as premeal insulin. The mealtime insulin dose should mirror...
271
Carbohydrate Metabolism
11.8K
Carbohydrates are polymers composed of molecules containing atoms of carbon, hydrogen and oxygen. One gram of carbohydrate can provide four kilo-calories of energy, which makes it the most efficient instant energy source.
Starch accounts for approximately 60% of the carbohydrates consumed by humans. Since amylase enzymes cannot function in the stomach's acidic environment, starch can only be digested in the mouth and small intestine. Simple sugars are found naturally in milk and fruits in...
Starch accounts for approximately 60% of the carbohydrates consumed by humans. Since amylase enzymes cannot function in the stomach's acidic environment, starch can only be digested in the mouth and small intestine. Simple sugars are found naturally in milk and fruits in...
11.8K
Diabetes: Management and Pharmacotherapy
412
The therapy for diabetes aims to alleviate hyperglycemia-related symptoms, prevent acute metabolic decompensation, and reduce chronic end-organ complications. Glycemic control is evaluated through short-term (self-monitoring, continuous glucose monitoring) and long-term (A1c, fructosamine) metrics, enabling near real-time tracking of blood glucose levels and reflecting glycemic control over specific time frames.
Insulin remains the cornerstone of treatment for most patients with type 1 and many...
Insulin remains the cornerstone of treatment for most patients with type 1 and many...
412
Diabetes Mellitus: Type 2 and Gestational
3.0K
Type 2 diabetes, characterized by insulin resistance, arises when the insulin receptors on cells lose responsiveness to insulin, diminishing the cell's capacity to take up glucose, resulting in elevated blood glucose levels. To receive a diagnosis of Type 2 diabetes, a series of blood glucose tests are necessary to assess whether the blood glucose falls within normal parameters. If the result is out of the normal range, a patient may be diagnosed as prediabetic or diabetic, depending on the...
3.0K
Diabetes Mellitus: Overview and Type I Subtype
3.3K
Diabetes mellitus is a chronic metabolic disorder characterized by high blood glucose levels due to inadequate insulin production, insulin resistance, or both. The condition affects millions worldwide and can significantly impact their health and quality of life.
Type 1 diabetes is an autoimmune disease in which the immune system mistakenly attacks and destroys the insulin-producing beta cells in the pancreas. As a result, the body is unable to produce sufficient insulin, and individuals with...
Type 1 diabetes is an autoimmune disease in which the immune system mistakenly attacks and destroys the insulin-producing beta cells in the pancreas. As a result, the body is unable to produce sufficient insulin, and individuals with...
3.3K
Oral Hypoglycemic Agents: Biguanides and Glitazones
310
Biguanides, particularly metformin (Glucophage), are insulin sensitizers that enhance glucose uptake, thereby reducing insulin resistance. Unlike sulfonylureas, metformin doesn't prompt insulin secretion, which helps to curb hypoglycemia risk. Metformin is beneficial in treating conditions like polycystic ovary syndrome due to its insulin-resistance reduction capability. The drug's primary action involves curtailing hepatic gluconeogenesis, a significant contributor to high blood...
310

