在早期慢性病中表征代谢失调,以获得诊断见解
Upasna Gupta1,2, Amrita Sahu1,2, Dharmendra Singh Bhadauria3
1Centre of Biomedical Research, Sanjay Gandhi Post Institute of Medical Sciences Campus, Raebareli Road, Lucknow, Uttar Pradesh, 226014, India. neerajcbmr@gmail.com.
Molecular omics
|May 29, 2025
概括
慢性病 (CKD) 的早期诊断是困难的. 这项研究使用代谢学识别了血清中的十个关键代谢物,为早期CKD检测和个性化治疗策略提供了潜在的生物标志物.
科学领域:
- * 生物化学和代谢学
- * 脏医学和诊断
背景情况:
- * 慢性病 (CKD) 在早期阶段 (G1-G3) 使用常规方法 (如血清肌素) 提出诊断挑战.
- * 早期发现和识别用于早期慢性病个性化治疗的生物标志物仍未得到充分研究.
- * 淋巴膜过率 (GFR) ≥30mL/min/1.73m2定义了早期CKD,表明功能正常到中度.
研究的目的:
- * 为了确定用于早期检测慢性病 (CKD) 的新生物标志物.
- * 调查与早期CKD相关的生理变化.
- * 探索早期CKD管理中个性化治疗策略的潜力.
主要方法:
- *使用质子核磁共振 (H NMR) 的代谢分析,对115个人血清样本 (24个健康对照组,91名早期CKD患者) 进行了代谢分析.
- *使用MetaboAnalyst 6.0进行的数据预处理和统计分析,包括PCA,PLS-DA,OPLS-DA,ANOVA和Wilcoxon Mann-Whitney测试.
- *随机森林建模用于CKD阶段差异化,KEGG数据库用于路径丰富,以及ROC分析用于代谢物诊断价值评估.
主要成果:
- *10种代谢物 (myo-Inositol,糖醇,pyruvate,carnitine,phenylalanine,tyrosine,histidine,TMAO,2-hydroxyisobutyrate,3-hydroxyisobutyrate) 在CKD各个阶段显示出显著的变化 (p < 0.05,VIP > 1).
- *接收器操作特征 (ROC) 曲线分析表明了诊断潜力,鉴定代谢物的AUC值大于0.7.
- *路径分析显示,内醇酸盐,氨酸,氨酸,氨酸,氨酸代谢,氨酸,氨酸,氨酸和氨酸的生物合成有显著的失调.
结论:
- * 这项代谢学研究成功地确定了早期慢性病 (CKD) 的潜在生物标志物.
- *在早期的CKD患者中观察到显著的代谢异常和途径失调.
- *这些发现支持开发个性化护理策略,以管理早期的CKD.
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