相关实验视频

Updated: Jun 16, 2025

Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity

Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity

Published on: January 15, 2014

黄色金枪鱼中Apo和DDT结合P-糖蛋白的冷EM结构

Megan A Young¹, Steven D Rees¹, Sascha C T Nicklisch²

¹Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0418, United States.

Environmental science & technology

|May 29, 2025

概括

像DDT这样的持久有机污染物会在海鲜中积累. 这项研究表明,DDT与金枪鱼P-糖蛋白结合,抑制其功能,并可能增加鱼类中的污染物水平.

科学领域:

环境科学环境科学
生物化学生物化学
海洋生物学海洋生物学

背景情况:

持久性有机污染物 (POP) 对海产品安全构成风险.
在食物链中海洋污染物转移的机制尚未完全理解.
像P-glycoprotein (P-gp) 这样的异生体载体保护生物体免受有毒化学物质的影响.

研究的目的:

为了研究DDT和黄鱼P-gp之间的分子相互作用.
了解DDT如何影响P-gp功能,并有助于海鲜中的生物积累.

主要方法:

使用冷电子显微镜 (cryo-EM) 来确定金枪鱼的结构P-gp.
分析了二二三乙烯 (DDT) 与 P-gp 载体的结合相互作用.

主要成果:

该研究显示,在没有基质的情况下,金枪鱼P-gp采用多种形状.
观察到DDT与P-gp的一种特定的,宽的向内变形结合.
这种结合相互作用表明一种抑制载体功能的机制.

结论:

结合金枪鱼P-gp的DDT可以抑制其运输活动.
这种抑制可能解释了在金枪鱼和其他海鲜中DDT的生物积累.
有机腐蚀物可以破坏食物链的各个层面的关键运输机制.

关键词:

美国ABC运输商ABC运输商杀虫剂DDT是一种杀虫剂.这是一种P-glycoprotein蛋白.膜传送器膜传送器海洋污染海洋污染黄金枪鱼是一种黄金枪鱼.

更多相关视频

Direct Cryosectioning of Drosophila Heads for Enhanced Brain Fluorescence Staining and Immunostaining

Direct Cryosectioning of Drosophila Heads for Enhanced Brain Fluorescence Staining and Immunostaining

Published on: February 7, 2025

The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells

The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells

Published on: February 27, 2020

相关概念视频

Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...