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转录全基因组测序在pPGCLCs诱导的分化阶段识别非编码RNA及其竞争的内源RNA网络

Zhan-Zhong Qiao¹, Qiu-Yuan Pang¹, Chang Xu¹

¹College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, 266109, China.

Stem cell reviews and reports

|May 29, 2025

概括

这项研究揭示了新型非编码RNAs调节猪的原始生殖细胞发育. 这些发现阐明了从干细胞诱导生殖细胞的分子机制,这对生殖生物学至关重要.

科学领域:

生殖生物学生殖生物学
干细胞生物学干细胞生物学
基因组学就是基因组学.

背景情况:

原始生殖细胞 (PGCs) 对于配子形成至关重要,它们的发展涉及复杂的基因调节.
非编码RNAs (ncRNAs) 在PGC分化中的作用在很大程度上仍未被探索.
了解PGC的发展对于推进生殖技术和保护工作至关重要.

研究的目的:

调查控制猪皮肤衍生干细胞 (pSDSCs) 与猪皮肤衍生干细胞 (pSDSCs) 差异化的ncRNA调控网络.
确定关键的ncRNA及其参与PGC细胞命运承诺的点.
阐明从pSDSCs诱导PGC类细胞 (pPGCLCs) 的基础分子机制.

主要方法:

区分pPGCLCs的整体转录组测序.
识别差异表达的微RNA (miRNA) 和长非编码RNA (lncRNA).
构建一个具有竞争力的内源RNA (ceRNA) 网络,并使用miRNA抑制剂和RT-qPCR进行实验验证.

主要成果:

涉及SMAD3和ACVR2B的TGF-β信号通路对于PGC命运承诺至关重要.
确定了一个包含特定miRNAs (ssc-miR-504, ssc-miR-125a, ssc-let-7c) 和lncRNAs (MSTRG.8397, MSTRG.5581, MSTRG.4342, MSTRG.4186, MSTRG.1058) 的ceRNA网络.
验证证实了在miRNA抑制时确定了lncRNAs和TGF-β通路基因的上调.

结论:

这项研究提供了ncRNAs在pSDSCs的pPGCLCs诱导过程中的第一个全面描述.
已识别的ncRNA和ceRNA网络为胚胎细胞发育的分子调节提供了新的见解.
这些发现有助于理解PGC诱导的机制,在干细胞研究和生物技术中具有潜在的应用.

关键词:

CeRNA网络 CeRNA网络的使用情况.在PGCLCs中,PGCLCs是最重要的.在TGF-β信号通路中.

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