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Updated: Jun 14, 2026

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在没有细胞内背景干扰的活细胞中可视化和定量化敏感的F-NMR

Chao-Yu Cui1, Bin Li1, Xing Zhang1

  • 1State Key Laboratory of Elemento-organic Chemistry, Tianjin Key Laboratory of Biosensing and Molecular Recognition, College of Chemistry, Nankai University, Tianjin 300071, China.

Journal of the American Chemical Society
|May 29, 2025
PubMed
概括

在活细胞中量化二氧化硫 (SO2) 是一个挑战. 使用P1-CF3探针的新型19F-NMR方法可以准确地实时可视化和测量各种哺乳动物细胞中的SO2水平.

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Chemical Shift: Internal References and Solvent Effects01:17

Chemical Shift: Internal References and Solvent Effects

In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...