相关实验视频
Updated: Sep 20, 2025

08:25
Identification of Circular RNAs using RNA Sequencing
Published on: November 14, 2019
12.4K
细胞质DIS3是一种外体独立的内核核酶,对循环RNA具有催化活性
Claudia Latini1, Julian Eichlinger1, Anna-Lisa Fuchs2
1Regensburg Center for Biochemistry (RCB), Laboratory for RNA Biology, University of Regensburg, 94053 Regensburg, Germany.
Cell reports
|May 29, 2025
概括
酶DIS3,一种核糖酶,可以降解细胞质中的圆形RNA (circRNAs). 这一发现表明DIS3在调节circRNA稳定性方面发挥着作用,独立于核外体.
科学领域:
- 分子生物学分子生物学
- 在RNA生物学,RNA生物学.
- 生物化学 生物化学
背景情况:
- 已知 ribonuclease DIS3 通过其 exoribonuclease 域降解线性 RNA,并与核外体相互作用.
- DIS3的PIN域具有内核酶活性,但其细胞基质在很大程度上仍未确定.
- 循环RNAs (circRNAs) 由于缺乏自由末端,其本质上是稳定的,这使得它们能够抵抗外核分解性降解.
研究的目的:
- 确定能够降解圆形RNA (circRNAs) 的核糖核酶.
- 研究DIS3在circRNAs降解中的作用.
- 为了确定DIS3是否在circRNA周转过程中独立于核外体的功能.
主要方法:
- 生物化学测试被用来选针对circRNAs的核核糖酶.
- 进行了细胞局部化研究,以评估DIS3在细胞质中的存在.
- 在体外测试了DIS3对各种circRNA的酶活性.
- 在细胞系中进行了 Knockdown 实验,以评估对 circRNA 稳定性的影响.
主要成果:
- DIS3被确定为circRNA降解的候选酶.
- 发现DIS3在细胞质中部分局部化,这是circRNA降解的地点.
- DIS3在体外表现出了对几种circRNA的分裂活性,这些circRNA的功能独立于外体细胞核.
- DIS3的淘汰导致细胞模型中特定circRNAs的适度稳定.
结论:
- 细胞质DIS3作为一个独立的酶,独立于外体细胞核,在circRNAs的降解中起作用.
- DIS3有助于circRNAs的周转,影响它们的细胞丰度.
- 这项研究揭示了DIS3在RNA代谢中的新功能,特别针对圆形RNA物种.
相关概念视频
mRNA Stability and Gene Expression
5.8K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
5.8K
Ribozymes
12.5K
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can...
Ribozymes can...
12.5K
RNA Interference
26.5K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.5K
RNA Splicing
57.2K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
57.2K
Nuclear Export of mRNA
7.9K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
7.9K
Experimental RNAi
6.3K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.3K

