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一个反意义RNA形成R循环,以促进CBF基因的转录和植物寒冷适应

Jianhang Sun¹, Xinyue Zhao¹, Diyi Fu²

¹Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China; Tsinghua University, Peking University Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.

Developmental cell

|May 29, 2025

概括

植物的寒冷适应包括C-REPEAT-BINDING FACTOR (CBF) 基因诱导. 一种天然的反意义长非编码RNA,CAS/SVK,增强了CBF1和CBF3的表达,提高了冷耐受性.

科学领域:

植物分子生物学植物分子生物学
转录法规转录法规
寒冷应激适应适应寒冷应激适应

背景情况:

植物通过诱导C-REPEAT-BINDING FACTOR (CBF) 基因来适应寒冷.
长非编码RNAs (lncRNAs),包括自然反意义转录 (NAT-lncRNAs),参与基因调节.
在Arabidopsis中,在CBF1和CBF3之间确定了一种特定的NAT-lncRNA,CAS/SVK.

研究的目的:

研究已识别的NAT-lncRNA (CAS/SVK) 在植物寒冷适应中的作用.
阐明CAS/SVK影响CBF基因表达和冷耐受性的机制.

主要方法:

在Arabidopsis中对lncRNAs和NAT-lncRNAs的全基因组注释.
在寒冷暴露后分析CAS/SVK表达水平.
研究CAS/SVK对CBF1和CBF3表达和植物冷耐受性的影响.
检查涉及R环形成和核细胞占用的分子机制.

主要成果:

寒冷暴露会迅速诱导CAS/SVK的表达.
CAS/SVK积极调节CBF1和CBF3的表达.
CAS/SVK通过组织R循环来促进CBF1和CBF3的转录,从而减少核细胞的占用率.
CAS/SVK赋予了植物获得的冷耐受性.

结论:

NAT-lncRNA CAS/SVK 在植物寒冷适应过程中起着至关重要的作用.
CAS/SVK促进了邻近的CBF基因的转录,增强了冷耐受性.
这项研究突出了lncRNA介导基因控制在植物应激反应中的重要性.

关键词:

在CAS/SVK中使用.在CBF的CBF中.这是一个R环.寒冷适应习惯的过程.基因转录基因转录在cnRNA中.

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