NLRP3蛋白转移到核中介 SV40 T-抗原诱导的角膜上皮细胞不朽化
Jie Xu1, Changkai Jia2, Qiaocheng Qiu3
1Eye Institute of Xiamen University, School of Medicine, Xiamen University, Xiamen, 361000, China.
Experimental eye research
|May 29, 2025
概括
核因子 (NF) -κB和富含白的重复蛋白3 (NLRP3) 在SV40 T-Ag介导的角膜细胞不朽化过程中转移到核中. NLRP3作为转录因子,影响RNA处理和病毒致癌.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 病毒学 病毒学
背景情况:
- NLRP3蛋白主要作为细胞质中炎症组分的功能.
- 在病毒感染或T细胞分化等特定条件下,NLRP3的核转位记录很少.
- 在诱导NLRP3核转位方面,SV40 T-抗原 (T-Ag) 的作用尚不清楚.
研究的目的:
- 调查SV40 T-Ag转染是否导致NLRP3核转位.
- 阐明由SV40 T-Ag引起的NLRP3核定位的机制和功能后果.
主要方法:
- 在人角膜上皮细胞 (HCECs) 中输送SV40 T-Ag.
- 核蛋白复合体拉下测试,然后进行质谱学.
- 生物信息分析包括集群和KEGG路径分析.
- 通过指数式丰富 (SELEX) 进行基因组的结构建模和系统演化,用于DNA基因的识别.
主要成果:
- SV40 T-Ag的诱导导致NLRP3转移到HCEC核中.
- 质谱学确定了285种与NLRP3相互作用的核蛋白,富含RNA结合,核细胞质运输和病毒致癌途径.
- 结构建模表明NLRP3和基因组子单元之间的相互作用.
- 在SELEX分析中,发现了与转录和RNA处理相关的由NLRP3结合的DNA基因.
结论:
- 在SV40 T-Ag介导的角膜细胞不朽化过程中,NLRP3转移到细胞核.
- 在核中,NLRP3的功能类似于转录因子,影响参与转录和RNA处理的基因.
- 这些发现揭示了NLRP3在非免疫细胞中的新功能,并有助于理解病毒介导的不朽化和致癌.
更多相关视频
05:22Author Spotlight: Characterizing DNA Replication of Pathogenic Repeats to Uncover Mechanisms of Replication Fork Stalling and Expansion
Published on: September 13, 2024
891
06:40Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy
Published on: November 4, 2018
6.3K
相关概念视频
01:03Rous Sarcoma Virus (RSV) and Cancer
5.4K
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
5.4K
01:45Regulation of Nuclear Protein Sorting
2.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.4K
03:18Non-LTR Retrotransposons
11.9K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.9K
03:08LTR Retrotransposons
18.0K
LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
18.0K
01:51Mechanisms of Retrovirus-induced Cancers
5.3K
Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
5.3K