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在口腔细胞中吸烟状态的表观遗传预测因子

Ewelina Pośpiech¹, Aleksandra Pisarek-Pacek², Kinga Herda³

¹Department of Genomics and Forensic Genetics, Pomeranian Medical University in Szczecin, Szczecin, Poland; Regional Center of Digital Medicine, Pomeranian Medical University in Szczecin, Szczecin, Poland.

Toxicology and applied pharmacology

|May 29, 2025

概括

在口腔细胞中的新DNA甲基化标记物可以识别吸烟暴露. 这些标记物,包括CYP1B1和DKK3中的新型标记物,为检测烟草使用提供了更高的准确性,并可以帮助卫生干预和法医分析.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子生物学分子生物学
公共卫生公共卫生

背景情况:

吸烟加快了全球的衰老和死亡率.
DNA甲基化标记对于了解吸烟的影响至关重要.
与吸烟相关的口腔细胞甲基化研究与血液相比是有限的.

研究的目的:

在口腔细胞中识别新型烟雾反应的DNA甲基化标记物.
为了比较口腔细胞标记物的有效性与已建立的血液标记物.
开发一种基于DNA甲基化的分类器,用于检测口腔表皮中的吸烟.

主要方法:

在200个个人的口腔细胞中对DNA甲基化进行表观基因组范围的关联分析.
对已识别的CpG标记物的功能分析.
后勤回归建模创建一个吸烟分类器.
在80个个体的独立队列中验证分类器.

主要成果:

在口腔细胞中确定了61个与吸烟相关的显著CpG标记物,包括新的位置.
发现AHRR,DKK3和CYP1B1基因是重要的标记物.
开发了一个四个CpG分类器 (CYP1B1,DKK3,AXIN1,PKN1) 的AUC=0.8,65.7%的灵敏度和81.5%的烟草使用的特异性.
在一个独立的样本集中验证了分类器.

结论:

状细胞DNA甲基化为吸烟暴露提供了宝贵的见解.
像CYP1B1和DKK3这样的新型标记物可能比口腔细胞中的传统标记物更具信息性.
开发的分类器可以帮助评估吸烟状态,干预和合规监测.

关键词:

行为概况分析 (Behavioral profiling) 是一种行为分析.口细胞是口细胞.在CYP1B1B1中.通过DNA甲基化.脂肪酸代谢脂肪酸代谢法医学 DNA 情报吸烟的推断是吸烟的推断.没有信号通路.

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