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基于PacBio和Hi-C技术的Paracoccus marginatus的染色体级基因组组合

Jiufeng Wei¹, Jinying Xue¹, Xuejie Shen¹

¹College of Plant Protection, Shanxi Agricultural University, Jinzhong, Shanxi, 030801, China.

Scientific data

|May 29, 2025

概括

番茄虫基因组被测序,为了解和管理这种入侵性害虫提供了至关重要的资源. 这种高质量的组件有助于人口遗传学和进化研究的研究.

科学领域:

基因组学就是基因组学.
昆虫学昆虫学是一门学科.
侵入性物种生物学生物学

背景情况:

侵袭性物种,如木瓜虫 (Paracoccus marginatus),在全球范围内带来了重大的生态和经济挑战.
帕拉科克 (Paracoccus marginatus) 是一种广泛的入侵性害虫,影响了60多个国家的200多种植物.

研究的目的:

为了组装侵袭性巴帕亚虫 (Paracoccus marginatus) 的染色体级基因组.
为研究入侵物种,种群遗传学和昆虫进化提供高质量的基因组资源.

主要方法:

用PacBio和Hi-C技术进行基因组测序.
用于基因组组装,注释和质量评估的生物信息分析 (BUSCO,CEGMA).

主要成果:

一个染色体级的基因组组合213.81 Mb,组织成四个染色体.
高连接 (20.2 Mb) 和脚手架 (48.01 Mb) N50值,表示连接组件.
基因组完整度为95.5% (BUSCO) 和99.56%的注释基因 (CEGMA),发现了13367个编码蛋白质的基因.
重复的序列占组装的基因组的49.26%.

结论:

高质量的P. marginatus基因组组合是推动入侵物种管理研究的关键资源.
这些基因组数据将有助于Hemiptera中的比较基因组学,并支持进化研究.
该组件为未来对害虫入侵性和适应性的遗传基础的研究提供了基础.

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Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.