相关实验视频

Updated: Sep 20, 2025

Expression of Transgenes in Native Bladder Urothelium Using Adenovirus-Mediated Transduction

Expression of Transgenes in Native Bladder Urothelium Using Adenovirus-Mediated Transduction

Published on: October 6, 2022

NEURL1通过降低PDE9A的调节作用,作为膀癌的候选抑制剂

Yu Qiu¹, Huijie Ruan², Decai Ji³

¹No. 1 Department of Urology, Cangzhou Central Hospital, Cangzhou, Hebei Province, China. qiuyu0317czszxyy@163.com.

In vitro cellular & developmental biology. Animal

|May 29, 2025

概括

神经化E3泛素蛋白联酶1 (NEURL1) 在膀癌中降低调节,并通过促进亡来抑制瘤生长. 过度表达NEURL1抑制了膀癌的进展和PDE9A水平.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物化学生物化学

背景情况:

膀癌是一种普遍存在的尿生殖系统恶性瘤.
在膀癌中神经化E3泛素蛋白联酶1 (NEURL1) 的作用仍未得到研究.
在细胞调节和疾病中,E3泛素连接酶起着至关重要的作用.

研究的目的:

研究NEURL1在膀癌中的表达和功能作用.
确定NEURL1对膀癌细胞增殖,细胞亡和进展的影响.
阐明NEURL1影响膀癌的分子机制.

主要方法:

在临床膀瘤样本中分析NEURL1表达.
在膀癌细胞系中稳定过度表达NEURL1及其RING域删除突变体 (5637和RT-112).
在NEURL1操纵后评估细胞生长,殖民地形成,细胞亡和蛋白质表达 (Ki-67,分裂的酶-3,PDE9A). 调查泛化和蛋白质体降解途径.

主要成果:

在临床膀瘤中,NEURL1的表达显著下调.
过度表达NEURL1抑制了膀癌细胞的生长,殖民地形成和Ki-67的表达.
过度表达NEURL1会增加细胞亡和分裂的caspase-3水平,这种影响取决于其RING域.
NEURL1促进了PDE9A的泛化和蛋白质体降解,导致PDE9A水平降低.
抑制PDE9A抑制了细胞活力,并增加了膀癌细胞的亡.

结论:

在膀癌中,NEURL1充当瘤抑制剂.
NEURL1通过诱导亡和抑制PDE9A.抑制膀癌的进展.
NEURL1代表了膀癌治疗的潜在治疗标.

关键词:

膀癌是一种癌症.在 NEURL1 中.在PDE9A中,我们可以使用PDE9A.在RING域名域名中.

更多相关视频

Evaluation of the Efficacy of the H. pylori Protein HP-NAP as a Therapeutic Tool for Treatment of Bladder Cancer in an Orthotopic Murine Model

Evaluation of the Efficacy of the H. pylori Protein HP-NAP as a Therapeutic Tool for Treatment of Bladder Cancer in an Orthotopic Murine Model

Published on: May 29, 2015

Identification of EGFR and RAS Inhibitors using Caenorhabditis elegans

Identification of EGFR and RAS Inhibitors using Caenorhabditis elegans

Published on: October 5, 2020

相关概念视频

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

Nonsense-mediated mRNA Decay

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...