新鲜抽出的母乳中的活力细菌群落及其在冷藏条件下的变化
Eliska Pivrncova1, Jan Bohm1, Vojtech Barton1
1RECETOX, Faculty of Science, Masaryk University, Kotlarska 2, Brno, Czech Republic.
International breastfeeding journal
|May 29, 2025
概括
将抽出的母乳存储在冰箱里长达48小时,可以保护活跃的细菌群落. 然而,冷母乳显著改变了细菌的组成和活力,影响了婴儿肠道微生物组的潜力.
科学领域:
- 微生物学 微生物学
- 人类牛奶中的寡糖化物
- 婴儿营养 婴儿营养
背景情况:
- 母乳含有各种细菌,对婴儿健康至关重要.
- 牛奶储存对活力的细菌组成的影响还不太清楚.
- 这项研究研究了在不同储存条件下抽出的母乳中可活性的细菌的变化.
研究的目的:
- 在新鲜抽出的母乳中描述活跃的细菌群落.
- 评估制冷和冷对这些细菌群落的影响.
主要方法:
- 收集了12名哺乳母亲的新鲜抽出的母乳.
- 在冷藏 (24h, 48h) 或冷 (6周) 下存储的样本.
- 使用 propidium monoazide (PMA) 与 16S rRNA amplicon 测序来识别可活性的细菌.
主要成果:
- 新鲜牛奶中常见的有生命力的细菌包括链球菌,葡萄球菌,Diaphorobacter,Cutibacterium和Corynebacterium.
- PMA治疗显著降低了测序深度和香农指数,表明选择性识别可活性的细菌.
- 冷显著改变了细菌的多样性和丰富性,特别是对于Diaphorobacter和Cutibacterium,而冷长达48小时却没有.
结论:
- 在人乳中,活力 (PMA 处理) 和总细菌之间的细菌特征显著不同.
- 长达48小时的冷藏对活跃的细菌群体的影响最小.
- 结显著影响母乳中的细菌的生存能力和组成.
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