环境DNA对基的吸附可以通过自然转换促进水平基因转移
Jacob D Holt1,2, Yixuan Peng1,2, Triana N Dalia3
1Department of Biological Sciences, Dartmouth, Hanover, NH 03755.
概括
霍乱病毒通过自然转化使用与素结合的DNA进行遗传交换. 基因吸收需要强力收回IV型 pili,突出显示素是基因转移的热点.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 水平基因转移 (HGT) 对细菌进化至关重要.
- 在Vibrio cholerae中,自然转化 (NT) 是由基表面诱导的.
- 环境DNA (eDNA) 的可用性是基因交换的关键.
研究的目的:
- 为了研究素结合DNA在Vibrio cholerae自然转化中的作用.
- 阐明从基表面吸收DNA的机制.
- 为了确定基表面作为HGT.潜在的热点.
主要方法:
- 使用光记者对活细胞进行成像.
- 细胞分辨率显微镜.细胞分辨率显微镜.
- 生理学上现实的实验条件.
主要成果:
- 自由的环境DNA吸附于基表面.
- 霍乱病毒利用与素结合的DNA作为基因交换的储存库.
- DNA吸收需要强制撤回能力类型IV pili.
结论:
- 素表面充当环境DNA的储存库.
- 类型IV pili的收缩力驱动NT期间的DNA吸收.
- 素颗粒作为热点用于Vibrio cholerae的水平基因转移.
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