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Updated: Jun 14, 2025

Induction and Analysis of Epithelial to Mesenchymal Transition
Published on: August 27, 2013
在舌头癌发生过程中,表皮细胞-介质细胞过渡和PD-1/PD-L1表达之间的双向相互作用
Hannah Gil de Farias Morais1, Helder Domiciano Dantas Martins2, Alexandre Rolim da Paz3
1Department of Oral Pathology, Federal University of Rio Grande do Norte, Natal, Brazil; Federal University of Rio Grande do Norte. Multicampi School of Medical Sciences, Caicó, Brazil.
表皮-介质细胞转变 (EMT) 在口腔舌状细胞癌 (OTSCC) 中很普遍,与免疫检查点蛋白PD-1和PD-L1相关. 这表明EMT可能会在OTSCC中驱动一种免疫抑制性瘤微环境.
科学领域:
- 在瘤学瘤学.
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- 口腔舌状细胞癌 (OTSCC) 是一种重要的头癌.
- 表皮介质转变 (EMT) 是一个细胞过程,与癌症的进展和转移有关.
- 免疫检查点蛋白,如PD-1和PD-L1,是抗瘤免疫反应的关键调节者.
研究的目的:
- 研究EMT与OTSCC中免疫检查点蛋白PD-1和PD-L1的表达之间的关联.
- 探索EMT状态,PD-1/PD-L1表达和OTSCC中的临床病理变量之间的关系.
- 确定EMT和PD-1/PD-L1表达对OTSCC患者生存的预后影响.
主要方法:
- 免疫组织化学被用来评估E-cadherin和N-cadherin表达,以确定EMT状态.
- 对PD-1和PD-L1免疫表达的半定量分析在61个OTSCC组织样本上进行.
- 进行了统计分析,以将EMT状态和PD-1/PD-L1表达与临床病理特征和生存率 (OS和DFS) 相关联.
主要成果:
- 在OTSCC中观察到阳性EMT状态 (75.4%) 的高患病率,与较高的组织学等级 (p=0.003) 显著相关.
- 增加PD-L1表达显著与较大的瘤大小相关 (p=0.048).
- 阳性EMT状态与PD-1 (p=0.003) 和PD-L1 (p=0.001) 的较高免疫表达相关,PD-1和PD-L1表达之间的中度阳性相关性 (r=0.571,p<0.001).
结论:
- 在OTSCC中,EMT是经常发生的事件,并且与PD-1和PD-L1.1的表达增加有关.
- 这些发现表明,EMT可能有助于OTSCC中免疫抑制瘤微环境的发展.
- 针对EMT或免疫检查点可能代表OTSCC的潜在治疗策略.
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