通过MALDI-MSI和LC-MS/MS,通过MALDI-MSI和LC-MS/MS,通过MALDI-MSI和LC-MS/MS,阐明单个西红中Alternaria毒素亚松酸的发生模式
Fan Sun1, Yingying Fan2, Yingying Dai2
1SINH-UGENT-SJTU Joint Laboratory of Mycotoxin Research, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Food chemistry
|May 30, 2025
概括
这项研究使用先进的成像绘制了西红中的十亚酸 (TeA) 地图,揭示了耐药西红线的内部真菌传播和毒素减少. 介绍了控制番茄TeA污染的新策略.
科学领域:
- 农业科学 农业科学
- 食品科学 食品科学 食品科学
- 菌类学 菌类学是指菌类学.
背景情况:
- 番茄 (Solanum lycopersicum) 易受Alternaria真菌的感染,导致酸 (TeA) 等毒素的污染.
- 之前的研究重点是整体TeA污染,缺乏在单个番茄内详细的空间分布分析.
研究的目的:
- 通过 MALDI-MSI 和 LC-MS/MS 来研究单个西红中的 TeA 的空间分布和平均水平.
- 要了解Alternaria alternata和TeA积累的感染动态.
- 为了识别番茄品种,提高了对A. alternata的抵抗力,并降低了TeA含量.
主要方法:
- 用于空间TeA分布的矩阵辅助激光消耗/电离质谱成像 (MALDI-MSI).
- 液体染色学-双重质谱学 (LC-MS/MS) 用于定量TeA分析.
- 人工接种番茄植物与Alternaria alternata.的人工接种.
主要成果:
- 在受损的加工番茄样本中观察到高TeA超标率.
- MALDI-MSI显示了与内部真菌殖民和外向病变相关的TeA分布模式.
- tgCotA番茄系表现出对A. alternata的显著耐药性和降低的TeA,而tgChitinase表现出增加的TeA.
结论:
- 内部真菌传播会影响西红中的TeA分布,内部度更高.
- 正如在tgCotA系列中所见的遗传耐药性,是减少TeA污染的可行策略.
- 这项研究为控制番茄和相关食品中的TeA提供了新的方法.
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