从 Plantago asiatica L. 提取的纯化多糖:制备,表征和免疫激活作用
Jiatong Zhang1, Wanbing Pan1, Jingwen Cui1
1National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; China Veterinary Medicine Innovation Center, China Agricultural University, Beijing 100193, China.
International journal of biological macromolecules
|May 30, 2025
概括
亚洲植物L.多糖 (PLP-I和PLP-II) 显示出强大的免疫调节作用. 这些化合物增强免疫细胞活性,减少瘤生长,并为功能性食物和免疫疗法提供了希望.
科学领域:
- 免疫学 免疫学 免疫学
- 自然产品 化学 化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 植物衍生的多糖因其免疫调节特性而闻名.
- 亚洲植物 (Plantago asiatica L.) 是一种具有潜在生物活性化合物的药用植物.
- 了解多糖的结构-活性关系对于开发新疗法至关重要.
研究的目的:
- 从 Plantago asiatica L. 中分离和描述多糖类.
- 研究这些多糖的免疫调节和抗瘤作用.
- 探索它们作为免疫治疗剂和功能性食品成分的潜力.
主要方法:
- 提取水和酒精沉以分离多糖.
- DEAE-52纤维素列色谱用于净化.
- 单糖化合物组成,甲基化,FT-IR和NMR分析用于结构阐明.
- 在体外对树突细胞成熟和功能进行测试.
- 在4T1瘤携带小鼠模型中的体内研究.
主要成果:
- 获得了两个多糖分,PLP-I (中性) 和PLP-II (酸性),并进行了结构性特征.
- 无论是PLP-I还是PLP-II都增强了树突细胞成熟标志物 (MHC-II,CD80,CD86) 并促进了IL-12的p70分泌.
- 在体内,多糖增加了CD4+,CD8+和CD11c+免疫细胞群和IFN-γ分泌.
- 在4T1瘤携带小鼠模型中,PLP-I和PLP-II显著降低了瘤重量.
结论:
- 亚洲植物L.多糖 (PLP-I和PLP-II) 具有显著的免疫调节和抗瘤活性.
- 这些多糖增强了先天性和适应性免疫反应.
- 它们显示出作为功能性食品成分和免疫治疗剂的潜力,可以促进免疫功能和对抗癌症.
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