由转录调节器RmaE激活的sRNA C263在酸性应激下调节细胞壁
Hao Wu1, Yue Zhang2, Zimo Zhao2
1Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, P. R. China; Zhejiang Research Institute of Tianjin University (Shaoxing), Shaoxin 312300, P. R. China.
Journal of dairy science
|May 30, 2025
概括
研究人员在Lactococcus lactis中发现了一种新的酸激活小RNA (sRNA),C263. 这种由RmaE调节的sRNA增强了细胞壁合成,为细菌应激反应和工业应用提供了洞察力.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 细菌生理学 细菌生理学
背景情况:
- 小RNAs (sRNAs) 是细菌对环境压力的反应的关键调节者.
- 乳球菌 (Lactococcus lactis) 在工业上很重要,但其sRNA调节机制,特别是关于在酸性压力下细胞壁平衡的机制,尚不清楚.
研究的目的:
- 为了识别和描述参与Lactococcus lactis中的酸性应激反应的新型sRNA.
- 阐明sRNA C263的调节机制及其在细胞壁酸甘合成中的作用.
- 研究转录调节器RmaE和sRNA C263.3之间的相互作用.
主要方法:
- 一种新的酸激活sRNA (C263) 的识别和特征.
- 对sRNA C263与目标mRNA (murA, murC, mraY) 的基配对相互作用的分析.
- 在体内验证转录调节器RmaE的sRNA C263激活,使用LacZ记者系统.
主要成果:
- 在L. lactis中发现了一种新的酸激活sRNA,C263.
- 通过向murA,murC和mRAYmRNAs,C263可以增强细胞壁丁糖甘的合成.
- 转录调节器RmaE通过与其促进体结合来激活C263的表达,从而显著增加β-galactosidase活性.
结论:
- 在酸性压力下,RmaE激活sRNA C263,这反过来又调节了L. lactis细胞壁稳态.
- 一个协同调节机制涉及转录调节器和sRNAs在酸性压力期间细胞壁的维护被证明.
- 了解这些机制对于推进工业L. lactis菌株的分子工程至关重要.
相关概念视频
Stringent Response in E. coli
300
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
300
Translational Regulation
544
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
544
Other Stress Responses in Bacteria
347
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
347
Types of RNA
72.6K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
72.6K
Types of RNA
9.1K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...
9.1K
Transcriptional Regulation: Riboswitches
592
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
592


