玻璃纤维接口的CRISPR/Cas生物传感器可适应各种生物标志物检测
Tian Yu1, Siyi Zou1, Yingxi Long1
1Integrated Devices and Intelligent Diagnosis (ID(2)) Laboratory, CUHK(SZ)-Boyalife Joint Laboratory of Regenerative Medicine Engineering, School of Medicine, The Chinese University of Hong Kong, Shenzhen, 518172, China.
Trends in biotechnology
|May 30, 2025
概括
一个新的生物传感平台,通用CRISPR/Cas与通用试剂设置 (g-CURS),可以检测各种生物标记物,如病毒核酸和帕金森病蛋白质,具有高灵敏度. 这项技术提供了快速,便携式的疾病检测,无需大型设备.
科学领域:
- 生物技术是生物技术.
- 分子生物学分子生物学
- 生物感应是一种生物感应.
背景情况:
- 开发用于多种生物标志物检测的敏感平台对于了解疾病和推进精准医学至关重要.
- 目前的方法可能缺乏多功能性或需要专门的设备.
研究的目的:
- 引入一个多功能,通用平台,用于多种生物标志物的超高灵敏度检测.
- 为了证明平台在检测核酸和蛋白质方面的能力.
主要方法:
- 开发了一种玻璃纤维接口的CRISPR/Cas系统,具有通用试剂设置 (g-CURS).
- 使用固定CRISPRRNA (crRNA) 和单链DNA (ssDNA) 激活器通过循环报道器 (CRs) 进行级联放大.
- 检测涉及的光信号被一个便携式探测器读取.
主要成果:
- 在30分钟内,g-CURS检测到具有心膜敏感性的病毒核酸.
- 在80分钟内检测到帕金森病血清中的低丰度蛋白质,具有亚皮科莫拉敏感性.
- 通过标准试剂设置,证明了简化的CRISPR/Cas生物感知.
结论:
- g-CURS为生物标志物检测提供了一个灵敏,快速和便携式的解决方案.
- 该平台对生物标志物发现和疾病诊断充满希望.
- 简化了CRISPR/Cas生物传感,减少了对大型仪器的依赖.
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