Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

1.3K
NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
1.3K
NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

935
A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
935
Applications Of NMR In Biology01:25

Applications Of NMR In Biology

4.0K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
4.0K
High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

1.6K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
1.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Coordination environments of Pt single-atom catalysts from NMR signatures.

Nature·2025
Same author

Bugs on Drugs: Paracetamol Exposure Reveals Genotype-Specific Generational Effects on Life History Traits in <i>Drosophila melanogaster</i>.

Insects·2024
Same author

Molecular Mechanisms of Temperature Tolerance Plasticity in an Arthropod.

Genome biology and evolution·2024
Same author

Simple MATLAB and Python scripts for multi-exponential analysis.

Magnetic resonance in chemistry : MRC·2024
Same author

Magnetic resonance free induction decay in geological porous materials.

The European physical journal. E, Soft matter·2021
Same author

Analytical Evaluation of Low-Field <sup>31</sup>P NMR Spectroscopy for Lipid Analysis.

Analytical chemistry·2019

相关实验视频

Updated: Sep 19, 2025

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the &#181;s-ms Timescale
08:09

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

Published on: April 19, 2021

5.4K

对于NMR设施的非专家用户的自动化数据处理工作流程.

Armin Afrough1, Maria Pérez-Mendigorri1, Thomas Vosegaard1,2

  • 1Interdisciplinary Nanoscience Center, Aarhus University, Aarhus, Denmark.

Magnetic resonance in chemistry : MRC
|May 31, 2025
PubMed
概括

自动化数据处理工作流程提高了核磁共振 (NMR) 光谱对各种科学用户的可访问性. 这些指导工具减少了专家的依赖,加快了分析,使复杂的NMR数据更容易管理.

科学领域:

  • 分析化学 分析化学
  • 生物物理学的生物物理.
  • 材料科学 材料科学 材料科学

背景情况:

  • 现代核磁共振 (NMR) 光谱仪昂贵而复杂,导致集中式超高场设施.
  • 来自各种科学领域的不同用户往往缺乏NMR专业知识,需要简化数据处理.
  • 现有的数据处理需要大量的专家参与或用户友好的自动化工作流程.

研究的目的:

  • 在超高场NMR设施中为广泛的用户社区开发自动化和指导数据处理工作流.
  • 为了提高NMR数据分析的用户友好性,稳定性和自动化.
  • 减少需要大量的NMR专家参与数据处理的需要.

主要方法:

  • 开发自动化和指导数据处理工作流程,包括一致性检查和中间结果报告.
  • 使用1H-31P TOCSY光谱,数据库匹配和定量31P测量的自动化定量脂肪学工作流程的实施.
  • 在食品矩阵中应用半自动化的多指数放松分析,使用L曲线来确定规范化参数.

主要成果:

  • 自动化工作流程成功地为广泛的用户社区提供服务,并减少了专家监督.
  • 脂管学工作流中的一致性检查和可靠性标签突出显示了潜在的数据陷.
  • 半自动放松分析有效地确定规范化参数,加速处理.
关键词:
自动化自动化自动化自动化数据分析数据分析数据分析核磁共振是一种核磁共振.

更多相关视频

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
07:24

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Published on: September 23, 2021

1.9K
NMR-Based Fragment Screening in a Minimum Sample but Maximum Automation Mode
09:19

NMR-Based Fragment Screening in a Minimum Sample but Maximum Automation Mode

Published on: June 4, 2021

3.5K

相关实验视频

Last Updated: Sep 19, 2025

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the &#181;s-ms Timescale
08:09

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

Published on: April 19, 2021

5.4K
Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
07:24

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Published on: September 23, 2021

1.9K
NMR-Based Fragment Screening in a Minimum Sample but Maximum Automation Mode
09:19

NMR-Based Fragment Screening in a Minimum Sample but Maximum Automation Mode

Published on: June 4, 2021

3.5K

结论:

  • 引导自动化显著减少了专家监督,并为各种用户加快了NMR数据处理.
  • 开发的工具提高了超高场NMR设施的可访问性和效率.
  • 未来的计划包括完善工作流程,开源软件共享,并探索新的应用领域.