相关实验视频

Updated: Jan 18, 2026

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Published on: August 16, 2018

在Blakeslea trispora中通过转录基因组分析三胺增强型烯积累的机制

Aizhong Han¹, Yue Liu¹, Ziang Gao¹

¹Lin He's Academician Workstation of New Medicine and Clinical Translation in Jining Medical University, Jining Medical University, Jining, China.

International microbiology : the official journal of the Spanish Society for Microbiology

|May 31, 2025

概括

三烯胺 (TPA) 治疗通过诱导DNA损伤和自增强了Blakeslea trispora中的烯生产. 这一过程涉及DNA修复,改变基因表达和细胞循环,导致利科储存增加.

科学领域:

微生物学微生物学
分子生物学分子生物学
生物化学生化学

背景情况:

利科是一种强大的抗氧化剂类胡卜素,由Blakeslea trispora生产.
三烯胺 (TPA) 显著提高了柳科的产量,但底层机制尚不清楚.

研究的目的:

为了阐明TPA增强B. trispora.中白烯生产的分子机制.
分析对TPA治疗反应的基因表达变化.

主要方法:

无参考转录组分析,以分析基因表达变化.
在TPA治疗后观察形态变化 (细胞胀,子数量).

主要成果:

治疗TPA导致了初级/二级代谢基因的下调和自和DNA修复基因的上调 (例如Xpb,Xpg,Csb,Polr2,Rmi1).
观察到菌体胀和减少子生产.
低调Lsm2和Dars2,高调G10,表明对mRNA结合酶体的影响.

结论:

TPA诱导DNA损伤,激活核酸切除修复通路.
细胞饥饿反应触发了自,回收子物质,并促进菌体的胀.
这些综合反应最终会增强B. trispora.中的白甘的产生和积累.

关键词:

布莱克斯莱亚三胞胎菌群 (Blakeslea trispora) 是一个自自是一种自的过程.这种药物是百合素 (Lycopene).没有参考的转录组.三烯胺是一种三烯胺.

更多相关视频

Translating Ribosome Affinity Purification TRAP to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

Translating Ribosome Affinity Purification TRAP to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

Published on: May 14, 2020

Genetic Incorporation of Biosynthesized L-dihydroxyphenylalanine DOPA and Its Application to Protein Conjugation

Genetic Incorporation of Biosynthesized L-dihydroxyphenylalanine DOPA and Its Application to Protein Conjugation

Published on: August 24, 2018

相关概念视频